Identification and Detection of ESBL Producing E.coli
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Urinary Tract Infections (UTIs) are the most common infections worldwide mainly caused by E.coli. Some of the E.coli organisms are able to produce an enzyme called Extended Spectrum Beta Lactamases (ESBLs) that cause high resistance to all beta-lactam antibiotics with the exception of carbapenems or cephamycins but inhibited by beta-lactam in combination with inhibitors like clavulanic acid, sulbactam and tazobactam. The main aim of this study was to determine the prevalence of ESBLs. Isolation and identification of ESBL producing E.coli isolates and its confirmation by various confirmative te...
Urinary Tract Infections (UTIs) are the most common infections worldwide mainly caused by E.coli. Some of the E.coli organisms are able to produce an enzyme called Extended Spectrum Beta Lactamases (ESBLs) that cause high resistance to all beta-lactam antibiotics with the exception of carbapenems or cephamycins but inhibited by beta-lactam in combination with inhibitors like clavulanic acid, sulbactam and tazobactam. The main aim of this study was to determine the prevalence of ESBLs. Isolation and identification of ESBL producing E.coli isolates and its confirmation by various confirmative tests. The study included total of 150 clinical specimens of urine and pus. The E.coli isolates which were screen positive were studied for ESBL detection by DDST and DDDT method. Out of 150 clinical samples 65 E.coli were isolates. Amongs them 28 E.coli isolates were screen positive for ESBL. Out of which only 13 E.coli isolates were ESBL producer and 15 E.coli isolates were non-ESBL producer. The ESBL percentage was more in female urine samples.
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