Hydrocarbon and Lipid Microbiology Protocols
Gebundenes Buch

Hydrocarbon and Lipid Microbiology Protocols

Microbial Quantitation, Community Profiling and Array Approaches

Herausgegeben: McGenity, Terry J.; Timmis, Kenneth N.; Nogales, Balbina
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This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput ...