Genetically Incorporated Non-Canonical Amino Acids
Gebundenes Buch

Genetically Incorporated Non-Canonical Amino Acids

Methods and Protocols

Herausgegeben: Tsai, Yu-Hsuan; Elsässer, Simon J.
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This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily ...