Genetic Diversity Analysis of Rice Bean Using Molecular Markers

Genetic Diversity Analysis of Rice Bean Using Molecular Markers

Molecular Profiling of Rice Bean (Vigna umbellata (Thunb.) Ohwi & Ohashi) Land Races Using RAPD and ISSR Markers

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Vigna umbellata (Thunb.) Ohwi and Ohashi commonly known as rice bean or climbing mountain bean is under exploited tropical legume, used as vegetable and fodder. Genetic variation between 10 landraces of rice bean was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. RAPD fingerprinting (70.30 %) detected more polymorphic loci than the ISSR fingerprinting (61.79%). Mean PIC (polymorphic information content) for each of these marker systems (0.243 for RAPD and 0.203 for ISSR) suggested that both the marker systems were equally effective in ...