From cell division to endoreduplication
Large-scale transcriptional profiling of the transition from cell division to endoreduplication in developing soybean seeds
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This work presents an early approach to large-scaletranscriptional profiling of the soybean seeddevelopmental transition from rapid cell division toendoreduplication. In the absence of soybean genomesequence a suppression-subtractive hybridizationapproach coupled with partial sequencing of theresulting differentially expressed transcriptsand subsequent custom microarray production was used.The resulting subset of transcripts specific to thedevelopmental process being studied was profiledusing cDNA microarrays as a time course from seven totwenty two days after flowering.
This work presents an early approach to large-scale
transcriptional profiling of the soybean seed
developmental transition from rapid cell division to
endoreduplication. In the absence of soybean genome
sequence a suppression-subtractive hybridization
approach coupled with partial sequencing of the
resulting differentially expressed transcripts
and subsequent custom microarray production was used.
The resulting subset of transcripts specific to the
developmental process being studied was profiled
using cDNA microarrays as a time course from seven to
twenty two days after flowering.
transcriptional profiling of the soybean seed
developmental transition from rapid cell division to
endoreduplication. In the absence of soybean genome
sequence a suppression-subtractive hybridization
approach coupled with partial sequencing of the
resulting differentially expressed transcripts
and subsequent custom microarray production was used.
The resulting subset of transcripts specific to the
developmental process being studied was profiled
using cDNA microarrays as a time course from seven to
twenty two days after flowering.
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