Feedback Tracking-FCS of Fluorescent Nanoparticles and Biomolecules
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The best way to study dynamic fluctuations in singlemolecules or nanoparticles is to look at only oneparticle at a time, and to look for as long aspossible. Brownian motion makes this difficult, asmolecules move along random trajectories that carrythem out of any fixed field of view.This thesis describes an instrument that tracks theBrownian motion of single fluorescent molecules inthree dimensions and in real-time while measuringfluorescence with nanosecond time resolution andsingle-photon sensitivity. The apparatus increasesobservation times by approximately three orders ofmagnitude while im...
The best way to study dynamic fluctuations in single
molecules or nanoparticles is to look at only one
particle at a time, and to look for as long as
possible. Brownian motion makes this difficult, as
molecules move along random trajectories that carry
them out of any fixed field of view.
This thesis describes an instrument that tracks the
Brownian motion of single fluorescent molecules in
three dimensions and in real-time while measuring
fluorescence with nanosecond time resolution and
single-photon sensitivity. The apparatus increases
observation times by approximately three orders of
magnitude while improving data-collecting efficiency
by locking tracked objects to a high-intensity region
of the excitation laser.
Applications of the instrument to the study of
quantum dot photon statistics and of the thermal
intramolecular motion of flexible polymer chains are
presented.
molecules or nanoparticles is to look at only one
particle at a time, and to look for as long as
possible. Brownian motion makes this difficult, as
molecules move along random trajectories that carry
them out of any fixed field of view.
This thesis describes an instrument that tracks the
Brownian motion of single fluorescent molecules in
three dimensions and in real-time while measuring
fluorescence with nanosecond time resolution and
single-photon sensitivity. The apparatus increases
observation times by approximately three orders of
magnitude while improving data-collecting efficiency
by locking tracked objects to a high-intensity region
of the excitation laser.
Applications of the instrument to the study of
quantum dot photon statistics and of the thermal
intramolecular motion of flexible polymer chains are
presented.
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