Embryonic Stem Cell-like Cells in Goat (Capra hircus)
Arun K. DeDhruba Malakar
Broschiertes Buch

Embryonic Stem Cell-like Cells in Goat (Capra hircus)

Derivation, Culture and Characterization

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The objective of the present experiment was production of goat embryos either by parthenogenetic activation or by in vitro fertilization and derivation of embryonic stem (ES) cell-like cells. Firstly, the effects of different activation methods, culture systems and culture media on in vitro development of zona-free and with-zona parthenogenetic embryos were investigated. Secondly, effect of cysteamine supplementation in the in vitro maturation (IVM) or culture (IVC) media, or a combination of both in terms of blastocyst production by in vitro fertilization was investigated. Finally, different ...