Electronic DNA Detection
Carbon Nanotube Field Effect Transistors
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Spurred by the Human Genome Project, massive genetic profiling of myriad diseases is being widely sought. Despite high throughput and sensitivity the conventional workhorses, i.e., micorarrays and bead-based assays involve complicated protocols and considerable expense due to the need for fluorescent labeling. Consequently, their utility is limited only to handful of well-endowed institutions. This work attempts a low cost alternative suitable for point-of-care facilities deployment. A method of using biochips based on carbon nanotube field effect transistor arrays is proposed and the feasibil...
Spurred by the Human Genome Project, massive genetic
profiling of myriad diseases is being widely sought.
Despite high throughput and sensitivity the
conventional workhorses, i.e., micorarrays and bead-
based assays involve complicated protocols and
considerable expense due to the need for fluorescent
labeling. Consequently, their utility is limited
only to handful of well-endowed institutions. This
work attempts a low cost alternative suitable for
point-of-care facilities deployment. A method of
using biochips based on carbon nanotube field effect
transistor arrays is proposed and the feasibility of
using these sensors to detect the presence of
specific DNA sequences, e.g. expressed genes, in a
solution of DNA or RNA is demonstrated. This book
provides an extensive review of current available
DNA detection schemes, a detailed description of the
carbon nanotube transistors, including modeling,
fabrication and experimental setup, as well as
results that demonstrate the efficacy for DNA
detection. A section is also included describing a
low-cost protein detection scheme based on gold
nanoparticle surface plasmon resonant absorption.
profiling of myriad diseases is being widely sought.
Despite high throughput and sensitivity the
conventional workhorses, i.e., micorarrays and bead-
based assays involve complicated protocols and
considerable expense due to the need for fluorescent
labeling. Consequently, their utility is limited
only to handful of well-endowed institutions. This
work attempts a low cost alternative suitable for
point-of-care facilities deployment. A method of
using biochips based on carbon nanotube field effect
transistor arrays is proposed and the feasibility of
using these sensors to detect the presence of
specific DNA sequences, e.g. expressed genes, in a
solution of DNA or RNA is demonstrated. This book
provides an extensive review of current available
DNA detection schemes, a detailed description of the
carbon nanotube transistors, including modeling,
fabrication and experimental setup, as well as
results that demonstrate the efficacy for DNA
detection. A section is also included describing a
low-cost protein detection scheme based on gold
nanoparticle surface plasmon resonant absorption.
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