Effects of Salivary and Digestive Gland extract on Angiogenesis
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Angiogenesis is the formation of new blood vessels from existing vessels. Natural inhibitor blocking the formation of new blood vessels is a promising anticancer or quick wound healing strategy. Chorioallontoic membrane (CAM) is well vascularized and used to study as invivo model of angiogenesis. In the present investigation the effect of salivary and digestive gland, alcohol and acetone extracts of Ariophanta pulmonata on angiogenesis especially CAM area, primary, secondary and tertiary vitelline veins was undertaken. Fertilized eggs of Gallus gallus were incubated at 380C and 70% relative hu...
Angiogenesis is the formation of new blood vessels from existing vessels. Natural inhibitor blocking the formation of new blood vessels is a promising anticancer or quick wound healing strategy. Chorioallontoic membrane (CAM) is well vascularized and used to study as invivo model of angiogenesis. In the present investigation the effect of salivary and digestive gland, alcohol and acetone extracts of Ariophanta pulmonata on angiogenesis especially CAM area, primary, secondary and tertiary vitelline veins was undertaken. Fertilized eggs of Gallus gallus were incubated at 380C and 70% relative humidity. In the embryos of 48, 55, 66, 72, 88 and 96 hrs CAM was exposed to 1mg/ml of different extracts and were further incubated up to 144 hrs and CAM was studied. Significant inhibitory effect of the salivary gland extract is evident. The inhibition of angiogenesis was highly significant at 48, 55 and 66 hrs of treated chick embryos. The anti-angiogenic role of complex carbohydrates form animal origin is discussed.
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