Dynamics of Bacteria-Mucus Interactions

Herausgegeben:Brockhausen, Inka

• Gebundenes Buch

Produktbeschreibung

This volume details the importance of bacteria in and around the human body, highlighting the bacteria that is harmless and pathogenic bacteria. Chapters guide readers through protocols on different bacteria s providing a better understand on they operate and how people can protect ourselves. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Dynamics of Bacteria-Mucus Interactions aims to be comprehensive guide for researchers in the field.

Produktdetails

• Methods in Molecular Biology 2942
• Verlag: Humana / Springer US / Springer, Berlin
• Artikelnr. des Verlages: 978-1-0716-4626-7
• Seitenzahl: 221
• Erscheinungstermin: 24. Juli 2025
• Englisch
• Abmessung: 254mm x 178mm
• ISBN-13: 9781071646267
• ISBN-10: 1071646265
• Artikelnr.: 73687485

Inhaltsangabe

Breaking Barriers: A Protocol to Investigate Intestinal Pathogen Adhesion, Invasion and Translocation Through In Vitro, In Vivo, and Imaging Technologies.- Quantitative assessment of MUC5AC and Helicobacter pylori adhesion to the gastric tissue on the model of Cavia porcellus.- Growth and Polarization of A549 Human Alveolar Epithelial Cells as a Model to Study Biofilm Formation in Acinetobacter baumannii.- Mucins as inhibitors against bacterial adhesion to epithelial cells.- Profiling of anti-glycan IgA and IgG in oral fluid using a glycan microarray.- Assays for Anti-Helicobacter pylori Effects of Lactic Acid Bacteria Secretions.- Assessing the relative affinities of bacterial lectins for sugars.- Investigating bacterial invasion of highly differentiated human bronchial epithelial cell barriers.- Methods of a reproducible mouse model of Clostridioides difficile infection to investigate novel bacterial based therapies.- Purification of Type IV and Type VI lipoteichoic acids from Gram-positive mucosal pathogens via a two-step hot water-phenol/triethylammonium acetate extraction method.- Microbial lectins targeting mucus: A glycobioinformatic approach.- Exploring the O-glycomic Degradome Using Natural Mucin Libraries.- Detection of Bacterial Adhesion to Mucus Slot-Blotted on Polyvinylidene Difluoride (PVDF) Membrane.- Quantification of -Lactamase Inhibition using a Luminescent Whole-Cell Biosensor.- Mucins and bacterial interactions.- Measuring and Analyzing Bacterial Movement in Mucus.- Adhesion of Pseudomonas aeruginosa to human outer layer airway mucins.