Developing Library-Based Bacterial Source Tracking Methods
Wail Hassan
Broschiertes Buch

Developing Library-Based Bacterial Source Tracking Methods

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The goal of this study is to develop efficient bacterial source tracking capabilities for the identification of sources of fecal pollution. Two polymerase chain reaction-based methods, BOX-PCR and REP-PCR, were compared to pulsed field gel electrophoresis. Additionally, source tracking fidelity was compared using two target organisms, Escherichia coli and Enterococcus spp. The highest fidelity of source tracking was achieved using BOX-PCR and Enterococcus spp. The study also showed that applying a similarity value (a measure of how similar an unknown isolate is to isolates from a known source)...