This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
1. Analyses of Subnanometer Resolution Cryo-EM Density Maps Matthew L. Baker, Mariah R. Baker Corey F. Hryc Frank DiMaio 2. Methods for Segmentation and interpretation of cryo-EM data Niels Volkmann 3. Integration of Cryo-EM with atomic and protein: protein interaction data Friedrich Forster 4. Unified Data Resource for CryoEM Catherine L. Lawson 5. Electron crystallography and aquaporins Thomas Walz, Andreas D. Schenk, Richard K. Hite, Andreas Engel, Yoshinori Fujiyoshi 6. Cryo-Electron Microscopy Applications in the Study of Tubulin Structure, Microtubule Architecture, Dynamics and Assemblies, and Interaction of Microtubules with Motors Kenneth H. Downin and Eva Nogales 7. Helical crystallization of two example membrane proteins: MsbA and the Ca2+-ATPase Howard S. Young, John Paul Glaves, Lauren Fisher, Andrew Ward 8. Multi-particle cryo-EM of ribosomes Christian M.T. Spahn, Justus Loerke, Jan Giesebrecht 9. Single-particle electron microscopy of animal fatty acid synthase: Describing macromolecular rearrangements that enable catalysis Francisco Asturias, Edward J. Brignole 10. Tomography of actin cytoskeletal networks Dorit Hanein 11. Visual Proteomics Friedrich Förster, Bong-Gyoon Han, and Martin Beck 12. Cryo-Electron Tomography of Eukaryotic Cells Ohad Medalia, Asaf Mader, Nadav Elad 13. 3d Visualization Of Hiv Virions By Cryo Electron Tomography Jun Liu, Elizabeth R. Wright, Hanspeter Winkler 14. Automation in Single-Particle Electron Microscopy: Connecting the Pieces Bridget Carragher, Dmitry Lyumkis, Arne Moeller, Anchi Cheng, Amber Herold, Eric Hou, Christopher Irving, Erica L. Jacovetty, Pick-Wei Lau, Anke Mulder, James Pulokas, Joel D. Quispe, Neil R. Voss, Clinton S. Potter
1. Analyses of Subnanometer Resolution Cryo-EM Density Maps Matthew L. Baker, Mariah R. Baker Corey F. Hryc Frank DiMaio 2. Methods for Segmentation and interpretation of cryo-EM data Niels Volkmann 3. Integration of Cryo-EM with atomic and protein: protein interaction data Friedrich Forster 4. Unified Data Resource for CryoEM Catherine L. Lawson 5. Electron crystallography and aquaporins Thomas Walz, Andreas D. Schenk, Richard K. Hite, Andreas Engel, Yoshinori Fujiyoshi 6. Cryo-Electron Microscopy Applications in the Study of Tubulin Structure, Microtubule Architecture, Dynamics and Assemblies, and Interaction of Microtubules with Motors Kenneth H. Downin and Eva Nogales 7. Helical crystallization of two example membrane proteins: MsbA and the Ca2+-ATPase Howard S. Young, John Paul Glaves, Lauren Fisher, Andrew Ward 8. Multi-particle cryo-EM of ribosomes Christian M.T. Spahn, Justus Loerke, Jan Giesebrecht 9. Single-particle electron microscopy of animal fatty acid synthase: Describing macromolecular rearrangements that enable catalysis Francisco Asturias, Edward J. Brignole 10. Tomography of actin cytoskeletal networks Dorit Hanein 11. Visual Proteomics Friedrich Förster, Bong-Gyoon Han, and Martin Beck 12. Cryo-Electron Tomography of Eukaryotic Cells Ohad Medalia, Asaf Mader, Nadav Elad 13. 3d Visualization Of Hiv Virions By Cryo Electron Tomography Jun Liu, Elizabeth R. Wright, Hanspeter Winkler 14. Automation in Single-Particle Electron Microscopy: Connecting the Pieces Bridget Carragher, Dmitry Lyumkis, Arne Moeller, Anchi Cheng, Amber Herold, Eric Hou, Christopher Irving, Erica L. Jacovetty, Pick-Wei Lau, Anke Mulder, James Pulokas, Joel D. Quispe, Neil R. Voss, Clinton S. Potter
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