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The characteristics of the healthcare reflect the importance of research efforts in both treatments and technologies to be conducted. In the healthcare, treatment, policy and planning decisions should be based on evidence (explicit knowledge) and experience (tacit knowledge) wherever possible, but research knowledge still under research. Initially, knowledge transfer (KT) aims to facilitate producing, accessibility and application of tacit and explicit knowledge and may provide solutions to this challenge. However, based on longitudinal and systematic reviews, robust research into the KT mechanism in healthcare is limited. This work proposes a conceptual framework of a multilevel process-based of enablers and barriers of KT mechanism. On the basis of extensive literature from many fields and mainly socio-technical model of Levite (1964), we moderate the KT mechanism into main elements related to characteristics of knowledge, source, and recipient. This work discusses how Knowledge transfer can be an emerging potential solution to encourage learning and distributing the knowledge which it can encounter the challenges of healthcare system.