CHEMICAL MECHANISM OF HOMOCITRATE SYNTHASE FROM Saccharomyces cerevisiae
jinghua qian
Broschiertes Buch

CHEMICAL MECHANISM OF HOMOCITRATE SYNTHASE FROM Saccharomyces cerevisiae

Versandkostenfrei!
Versandfertig in 6-10 Tagen
44,99 €
inkl. MwSt.
PAYBACK Punkte
22 °P sammeln!
Homocitrate synthase (HCS1) (acetyl-coenzyme A:2-ketoglutarate C-transferase; E.C. 2.3.3.14)catalyzes the first and regulated step in thea-aminoadipate pathway for lysine synthesis. The pH dependence of the kinetic parameters, isotopeeffects, and dissociation constants for competitiveinhibitors are used to probe the chemical mechanismof HCS. A general acid-base chemical mechanism isproposed. site-directed mutagenesis was used tochange the three active site residues of HCS, and theresulting mutant enzymes were characterized usinginitial velocity studies, the pH dependence of thekinetic paramete...