CHARACTERIZE MAMMALIAN TRANSCRIPTOME COMPLEXITY
Jiaqian Wu
Broschiertes Buch

CHARACTERIZE MAMMALIAN TRANSCRIPTOME COMPLEXITY

FROM GENOME-WIDE RT-PCR CDNA CLONING, TO NOVEL MAMMALIAN GENE DISCOVERY, AND TRANSCRIPTOME COMPLEXITY

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In my Ph.D. thesis, I described a large scale RT-PCR and cloning pipeline that I developed to obtain several thousand full-length mammalian (mainly human and mouse) cDNA clones for the NHGRI/NCI Mammalian Gene Collection effort. At that time, the layers of the mammalian transcriptome complexity were not yet fully conceived. In this project, I was deeply involved in strategic and conceptual issues in characterizing transcriptome complexity from different levels. Using the RT-PCR pipeline combined with gene prediction algorithms that utilize comparative genomic information, we successfully ident...