Biological analysis of DNA 1 using as a silencing/expression vector

Biological analysis of DNA 1 using as a silencing/expression vector

Molecular Characterization and the potential use of begomovirus associated DNA 1 as a silencing/expression vector

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The work presented in this book consists of three disparate elements which have the unifying feature of being concerned with alphasatellite. The satellite-like alphasatellites (previously known as DNA 1) were first identified in 1999 and were shown to be associated with the majority of begomoviruses (family Geminiviridae) that require the satellite molecule now collectively known as betasatellites. Virus-induced gene silencing (VIGS) is a powerful reverse genetics tool for application in functional genomics. The alphasatellite-based VIGS vector was shown to be functional by silencing expressio...