Bioconversion of Agricultural Cellulose Waste to Usable Product
Versandkostenfrei!
Versandfertig in 6-10 Tagen
24,99 €
inkl. MwSt.
PAYBACK Punkte
12 °P sammeln!
Cellulolytic nitrogen fixing bacteria were isolated from different soil sources on nitrogen free cellulose mineral media. After determining their dual activities which were enhanced by transposon mutagenesis using recombinant E.coli S17 carrying cellulase gene from Rhizobium leguminosarum. The targeted strains that carry the transposon were selected on 2 x YT media which contains chloramphenicol (0.6 mg/20ml) and kanamycin (0.4mg/20ml). Nitrogen fixing activity was detected by ammonium test kit. The nitrogen fixing activities of wild type and mutant strains of Alcaligenes sp. and Azomonas agil...
Cellulolytic nitrogen fixing bacteria were isolated from different soil sources on nitrogen free cellulose mineral media. After determining their dual activities which were enhanced by transposon mutagenesis using recombinant E.coli S17 carrying cellulase gene from Rhizobium leguminosarum. The targeted strains that carry the transposon were selected on 2 x YT media which contains chloramphenicol (0.6 mg/20ml) and kanamycin (0.4mg/20ml). Nitrogen fixing activity was detected by ammonium test kit. The nitrogen fixing activities of wild type and mutant strains of Alcaligenes sp. and Azomonas agilis were almost the same. Cellulolytic activity of was detected by both plate screening method and by Dinitrosalicylic colorimetric (DNS) method. Cellulolytic activity of mutant strains was enhanced and they gave higher activities. The effectiveness of the selected strains was studied in composting process and complete composting was obtained at 4th week.
Andere Kunden interessierten sich für
