Bacterial Secretion Systems
Broschiertes Buch

Bacterial Secretion Systems

Methods and Protocols

Herausgegeben: Journet, Laure; Cascales, Eric
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This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introducti...