Assessment of the Methods for the Detection of Virus-Related Grapevin
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Grapevine, an important crop in many areas of the Taiwan, is affected by a number of diseases.Grapevine yellow speckle viroid-1 (GYSVd-1) and hop stunt viroid (HSVd) are two viroids known to infect naturally grapevines. In Taiwan, grape cultivation is a remunerative agri-business for the highly-popular grape berries among local consumers in addition to the added values with the processed products. The annual production of grapes was 102.831 metric tons in 2010, and 99.5% was produced in Taichung City, Miaoli, Changhua and Nantou Counties in Central Taiwan. Gene sequences were available in at t...
Grapevine, an important crop in many areas of the Taiwan, is affected by a number of diseases.Grapevine yellow speckle viroid-1 (GYSVd-1) and hop stunt viroid (HSVd) are two viroids known to infect naturally grapevines. In Taiwan, grape cultivation is a remunerative agri-business for the highly-popular grape berries among local consumers in addition to the added values with the processed products. The annual production of grapes was 102.831 metric tons in 2010, and 99.5% was produced in Taichung City, Miaoli, Changhua and Nantou Counties in Central Taiwan. Gene sequences were available in at the National Center for Biotechnology Information (NCBI), and gene-specific primers for RT-PCR were designed using the Clustal-W software. The method was validated by testing 50 grapevine samples from Taiwan and showed reliability and high sensitivity and was developed for the simultaneous detection of two grapevine viroids.
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