Application of Immobilized Laccase in Bioreactor

Application of Immobilized Laccase in Bioreactor

from Local Isolate of Pseudomonas aeruginosa SR3

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Immobilization of enzyme was conducted in two methods, entrapment by calcium alginate and agar-agar with maximum immobilization efficiency 49% and 32%, respectively and covalent method by chitosan and gelatin with maximum immobilization efficiency 63% and 50% respectively. Decolorization of black textile dye was performed also by operation of packed bed bioreactor continuously at different flow rates (15, 30 and 45) ml/hr and HRT (7.9, 3.9 and 2.6) hr. respectively. The black textile dye was loaded over the chitosan immobilized laccase with total working volume of 118 ml at concentration of (3...