Antiviral Activity of Acaccia Nilotica
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Methanol, chloroform and water extracts of Acacia nilotica sub species tomentosa were tested in-vitro for their potential antiviral activity against the Komarov vaccine strain of Newcastle disease virus (NDV). Sub culturing the treated samples and heamagglutination test were used to evaluate the reduction of viability in the viral growth in presence and absence of the effective extract. The noncytotoxic concentration of each extract was determined by the absence of the CPE. The inhibitory concentration of the effective extract was measured. The tannin percentage of the methanol and water extra...
Methanol, chloroform and water extracts of Acacia nilotica sub species tomentosa were tested in-vitro for their potential antiviral activity against the Komarov vaccine strain of Newcastle disease virus (NDV). Sub culturing the treated samples and heamagglutination test were used to evaluate the reduction of viability in the viral growth in presence and absence of the effective extract. The noncytotoxic concentration of each extract was determined by the absence of the CPE. The inhibitory concentration of the effective extract was measured. The tannin percentage of the methanol and water extracts were evaluated by Prussian Blue assay. The methanol extract of Acacia nilotica on vero cells showed non- cytotoxic concentration of 40mig/ml. The methanol extract showed a significant inhibitory effect against the tested virus at the concentration of 40 mig/ml. None of the other two extracts showed any inhibitory activity. Subculturing of the treated samples of the methanol extract showed no CPE compared to the control. The HA test showed zero titer of the tested samples against 2 log 6 to the control.
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