ANEW Pronase Inducible Lectin in Human serum

ANEW Pronase Inducible Lectin in Human serum

Detection, Isolation, Characterisation and Analyses of Immune Function of a Protease-Inducible Lectin In Human serum

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This investigation was aimed at exploring the possibility to generate defense molecules, such as lectins and phenoloxidase in human serum upon treatment with various exogenous proteases and detergents in vitro. Generation of phenoloxidase and lectin activity was detected, for the first time, upon treatment of human serum with exogenous elicitors. The lectin molecules generated in pronase-treated human serum was successfully isolated by a single step lectin affinity chromatography using concanavalin A- Sepharose 4B as affinity matrix and 200 mM, mannose in tris-HCl buffer (100 mM, pH 8.5) as an...