Analysis of Neuronal Microcircuits and Synaptic Interactions
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Main description:This volume of the Handbook of Chemical Neuroanatomy describes the techniques used to study the connectivity of chemically defined neurons at the cellular level. Among areas dealt with are the most recent developments in light microscopy and electron microscopy, and the applications to specific subjects of chosen combinations of techniques to demonstrate how elegantly and powerfully they can be applied.As the fiber connections of the brain become increasingly accurately mapped, the desire to understand the instrinsic circuits of these populations of neurons, including their mo...
Main description:
This volume of the Handbook of Chemical Neuroanatomy describes the techniques used to study the connectivity of chemically defined neurons at the cellular level. Among areas dealt with are the most recent developments in light microscopy and electron microscopy, and the applications to specific subjects of chosen combinations of techniques to demonstrate how elegantly and powerfully they can be applied.
As the fiber connections of the brain become increasingly accurately mapped, the desire to understand the instrinsic circuits of these populations of neurons, including their morphology, neurotransmitters, neuromodulators, receptors and their physiology, will grow. It is this desire for knowledge the authors are attempting to stimulate by presenting a comprehensive technical book on most of the currently used and theoretically possible combinations of anatomical, physiological and immunocytochemical methods for the study of neuronal micricircuits and synaptic interaction. An indispensible guide and "laboratory benchtool".
Table of contents:
I. Characterization and use of multi-color fluorescence microscopic tecnhiques (M.W. Wessendorf). II. Light and electron microscopic tracing of neuronal connections with Phaseolus vulgaris-leucoagglutinin (PHA-L), and combinations with other neuroanatomical techniques (H.J. Groenewegen and F.G. Wouterlood). III. Combined morphological and histochemical techniques for the study of neuronal microcircuits (J.P. Bolam and C.A. Ingham). IV. Synaptic interaction between chemically defined neurons: dual ultrastructural immunocytochemical approaches (A.N. van den Pol and C. Decavel). V. Intracellular injection of neurons in fixed brain tissue combined with other neuroanatomical techniques at the light and electron microscopic level (E.H. Buhl, W.K. Schwerdtfeger and P. Germroth). VI. Immunocytochemical identification of electrophysiologically characterized cells (K.G. Smithson and G.I. Hatton). VII. Anatomical analysis of electrophysiologically characterized neurons is the rat strio-pallidal system (H.T. Chang and C.J. Wilson). VIII. Convergence of morphological, physiological and immunocytochemical techniques for the study of single Mauthner cells (H. Korn, D.S. Faber and A. Triller). IX. In situ hybridization histochemistry (W.S. Young III). Subject index.
This volume of the Handbook of Chemical Neuroanatomy describes the techniques used to study the connectivity of chemically defined neurons at the cellular level. Among areas dealt with are the most recent developments in light microscopy and electron microscopy, and the applications to specific subjects of chosen combinations of techniques to demonstrate how elegantly and powerfully they can be applied.
As the fiber connections of the brain become increasingly accurately mapped, the desire to understand the instrinsic circuits of these populations of neurons, including their morphology, neurotransmitters, neuromodulators, receptors and their physiology, will grow. It is this desire for knowledge the authors are attempting to stimulate by presenting a comprehensive technical book on most of the currently used and theoretically possible combinations of anatomical, physiological and immunocytochemical methods for the study of neuronal micricircuits and synaptic interaction. An indispensible guide and "laboratory benchtool".
Table of contents:
I. Characterization and use of multi-color fluorescence microscopic tecnhiques (M.W. Wessendorf). II. Light and electron microscopic tracing of neuronal connections with Phaseolus vulgaris-leucoagglutinin (PHA-L), and combinations with other neuroanatomical techniques (H.J. Groenewegen and F.G. Wouterlood). III. Combined morphological and histochemical techniques for the study of neuronal microcircuits (J.P. Bolam and C.A. Ingham). IV. Synaptic interaction between chemically defined neurons: dual ultrastructural immunocytochemical approaches (A.N. van den Pol and C. Decavel). V. Intracellular injection of neurons in fixed brain tissue combined with other neuroanatomical techniques at the light and electron microscopic level (E.H. Buhl, W.K. Schwerdtfeger and P. Germroth). VI. Immunocytochemical identification of electrophysiologically characterized cells (K.G. Smithson and G.I. Hatton). VII. Anatomical analysis of electrophysiologically characterized neurons is the rat strio-pallidal system (H.T. Chang and C.J. Wilson). VIII. Convergence of morphological, physiological and immunocytochemical techniques for the study of single Mauthner cells (H. Korn, D.S. Faber and A. Triller). IX. In situ hybridization histochemistry (W.S. Young III). Subject index.