Allele-specific Oligonucleotide
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Allele-specific Oligonucleotide

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High Quality Content by WIKIPEDIA articles! An allele-specific oligonucleotide is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay. It is a common tool used in genetic testing, forensics, and Molecular Biology research. An ASO is typically an oligonucleotide of 15u201321 nucleotide bases in length. It is designed in a way that makes it specific for only one version, or allele, of the DNA being tested. The length of the ASO, which stra...