A Novel Cold Active Esterase from the Marine Psychrotolerant Isolate Pseudoalteromonas Arctica sp. nov.
Rami Al Khudary
Broschiertes Buch

A Novel Cold Active Esterase from the Marine Psychrotolerant Isolate Pseudoalteromonas Arctica sp. nov.

Versandkostenfrei!
Versandfertig in 1-2 Wochen
25,00 €
inkl. MwSt.
PAYBACK Punkte
0 °P sammeln!
In recent years, growing attention has been devoted to cold adapted microorganisms and their enzymes. A gene library was constructed using the genome of a new aerobic psychrotolerant marine isolate Pseudoalteromonas arctica sp. nov. The gene encoding an esterase was identified by screening the library on tributyrin plates. The recombinant protein was purified (3,3 - fold) in one step using a Ni- column and was subsequently characterised. The enzyme has unique properties since it requires a very low activation energy and retains 50% of its maximal activity at the freezing point of water.