The Efficiency of Rabbits Transgenesis by Sperm Mediated Gene Transfer
The feasibility of sperm cells to act literally as gene vehicles
was discussed using one of the most modern techniques in this
aspect which is known as restriction enzyme mediated integration
sperm mediated gene transfer (REMI-SMGT)technique. Several
objectives were pursued in this book; such as to test the validity
of REMI-SMGT, to evaluate the efficiency of this technique in
generating mammalian species rather than the sheep that made only
by one group and to observe the possibility of doing so by using
rabbits as a model for this approach. Add to that, it was aimed to
reduce the cost of REMI-SMGT by substituting liposomes and highly
cost effective media with a high efficient, non-cost effective
substitute. Moreover, it was directed to identify if any band
substitution was occurred by simple seminal proteins - exogenous
DNA incubation by one gel double stained non-radioactive method to
identify the role of seminal proteins charge on the nature of
seminal fluid inhibitory effect that exerted against the invading
DNA. This might help us to approach closely to the nature of
inhibitory activities in seminal proteins that prevent the entry of
foreign DNA into the head of the sperm.
Mohammed Baqur S. A. Al-ShuhaibBirth date: 1979 - IraqBsc. biology / University of Babylon (first of all)-2001Msc. biotechnology / University of Baghdad (first of all)-2005PhD. Biology - biotechnology / University of Babylon (first of all)-2011Academic member: College of Biotechnology/Al-Qasim Green UniversitySpecialty: Genetic Engineering.
