Genetic Engineering and Molecular Studies of Insulin Secreting Cells
This book reveals some details of research done at Marshall
University, USA, involving functional aspects of pancreatic beta
cells in several experimental models: avian, transgenic mice, and
beta-cell lines. In this book you will gain some knowledge of
genetic engineering approaches to improve beta-cell insulin
secretion which could be applied to manage models and cases of
diabetes mellitus. This work is presented as three separate
chapters. The first chapter aims to gain an understanding of the
expression pattern of CaBP28k in the chicken pancreas and to
explore its spatial relationships with insulin in beta cells. The
second chapter is to identify the influence of CaBP28k-ablation on
the dynamics of [Ca2+]i in response to glucose stimulation in
murine beta cells of CaBP28k-KO mice pancreatic islets and wildtype
islets. The third chapter is an attempt to elucidate the genomic
and non-genomic effects of in vitro CaBP28k-ablation in a cultured
beta-cell line. It highlights the effects on the gene expression
profile and insulin secretory responses from cultured rat
insulinoma cell lines using antisense oligonucleotides
transfection.
AYMAN S. AMER, MD, PhD: Studied Biomedical Sciences at Marshall University, USA. Associate Professor, Oman Medical College, P.O. Box 391 Al-Tareef, PC 321 Sohar, Oman. (00968) 95169546. amer1@marshall.edu, previously worked as research fellow at Marshall University then as Associate Professor at University of Missouri- Kansas City, USA.
