Stephen Sullivan (ed.) / Chad Cowan / Kevin Eggan

Human Embryonic Stem Cells

The Practical Handbook
Herausgegeben:Sullivan, Stephen; Cowan, Chad; Eggan, Kevin

• Gebundenes Buch

Produktbeschreibung

Since their isolation in 1998, human embryonic stem (ES) cells have been used as a powerful experimental model for studying the mechanisms of stem cell self-renewal, pluripotency, and differentiation in humans. However, the protocols by which these cells are grown still differ considerably from laboratory to laboratory and standardisation is needed in this young area of research.

Human Embryonic Stem Cells: The Practical Handbook contains all basic methods for sourcing, deriving, culturing, and manipulating human ES cells. The editors have gathered the leading scientists in the field to describe the protocols that they have carried out and tested in their own labs.

The book
_ Contains practical information for newcomers to the field: from how to source human ES cells from international cell banks to organizing the laboratory to accommodate work with these cells.

_ Provides protocols for routine culture as well as specialized protocols for directing differentiation into specific cell lineages.

_ Addresses the different proclivities and behaviours of individual human ES cell lines: authors state which lines they tried their protocols on and, more importantly, which lines worked best.

_ Includes troubleshooting sections that address common problems and offer advice on how these can be overcome.

_ Follows a simple, concise, and consistent structure: background material and hypothesizing are kept to a minimum to insure clarity and usability at the bench.

Human Embryonic Stem Cells: The Practical Handbook is the essential source of up-to-date, practical information for all researchers and clinicians working with human embryonic stem cells.

Produktdetails

• Verlag: Wiley & Sons
• Artikelnr. des Verlages: 14503356000
• 1. Auflage
• Seitenzahl: 424
• Erscheinungstermin: 1. August 2007
• Englisch
• Abmessung: 253mm x 177mm x 29mm
• Gewicht: 950g
• ISBN-13: 9780470033562
• ISBN-10: 0470033568
• Artikelnr.: 22616774

Autorenporträt

Stephen Sullivan. Harvard University, Cambridge, MA, USA Kevin Eggan. Harvard University, Cambridge, MA, USA Chad A. Cowan. Harvard University, Cambridge, MA, USA

Inhaltsangabe

Foreword. Davor Solter Preface. Section 1: Obtaining and culturing human
embryonic stem cells. 1 Organization and good aseptic technique in the
human embryonic stem cell laboratory. Minal J Patel, Emma L Stephenson, and
Stephen L Minger 2 Sourcing human embryonic stem cell lines. Glyn Stacey 3
Human embryo culture for human embryonic stem cell derivation. R. Douglas
Powers and Jeannine Witmyer 4 Derivation of human embryonic stem cell
lines. Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton,
Kevin Eggan and Chad A. Cowan 5 Standard culture of human embryonic stem
cells. Jeremy M. Crook, Rachel Horne, and Alan Colman 6 Culture of human
embryonic stem cells in chemically defined conditions. Julie Hsu Clark and
Sheng Ding Section 2: Characterization of undifferentiated embryonic stem
cells. 7 Phenotypic analysis of human embryonic stem cells. Jonathan S.
Draper, Cheryle A. Séguin and Peter W. Andrews 8 Genetic and epigenetic
analysis of human embryonic stem cells. Laurie A. Boyer, Rudolf Jaenisch,
and Maisam Mitalipova Section 3: Manipulation of human embryonic stem
cells. 9 In vivo differentiation of human embryonic stem cells. Scott A.
Noggle, Francesca M. Spagnoli, Ali H. Brivanlou 10 In vitro differentiation
of human embryonic stem cells. A. Henry Sathananthan and Alan Trounson 11
Differentiation of human embryonic stem cells into extraembryonic cell
types. Cheryle A. Séguin and Jonathan S. Draper 12 Directed differentiation
of human embryonic stem cells into: (a) Early endoderm cells. Kenji
Osafune, Alice E. Chen and Douglas A. Melton (b) Hepatic cells. Neta Lavon
and Nissim Benvenisty (c) Pancreatic cells. Hiram Chipperfield 13 Directed
differentiation of human embryonic stem cells into: (a) Cardiomyocytes.
Christine Mummery, Robert Passier and Chris Denning (b) Endothelial cells.
Carrie Soukup, Shulamit Levenberg and Ondine Cleaver (c) Osteogenic cells.
Jeffrey M. Karp, Alborz Mahdavi, Lino S. Ferreira, Ali Khademhosseini and
Robert Langer (d) Hematopoietic in vivo repopulating cells. Shannon
McKinney-Freeman, Thorsten M. Schlaeger and George Q. Daley (e)
Lymphocytes. Petter S. Woll and Dan S. Kaufman (f) Myeloid cells. Chantal
Cerdan and Mickie Bhatia 14 Directed differentiation of human embryonic
stem cells into: (a) Forebrain neurons. Emily A. Davis and Lawrence S.B.
Goldstein (b) Dopaminergic neuron. Jan Pruszak and Ole Isacson (c) Spinal
motor neurons. Bao-Yang Hu and Su-Chun Zhang 15 (a) Gene targeting: knock
out and knock in by homologous recombination. Thomas P. Zwaka (b) RNA
interference in human embryonic stem cells. M. William Lensch, Asmin
Tulpule and Holm Zaehres (c) Generation of gene reporters using bacterial
artificial chromosome recombineering. Andrew J. Washkowitz and David A.
Shaywitz Afterword. Azim Surani Index.

Rezensionen

"...the imaginative solutions in this book can inspire us to get past our most frustrating limitations."
Cell Stem Cell

"...the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human ES cells..."
Future Medicine

"In this fast-moving field, this [handbook] will help drive advances of more and more researchers." Differentiation

"... the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human pluripotent stem cells..." REGENERATIVE MEDICINE

"......a valuable resource for seasoned and novice researchers. ...an excellent addition to any...medical library or research laboratory."
THE AMERICAN MEDICAL ASSOCIATION