This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field. While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and organ form and function. Written for the highly successful Methods in Molecular Biology series,…mehr
This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field. While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and organ form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Decellularized Scaffolds and Organogenesis: Methods and Protocols share novel approaches and insights that will provide new opportunities for those already in the field or moving to enter the field.
Decellularization Methods for Scaffold Fabrication.- Decellularized Liver Scaffold for Liver Regeneration.- Wharton's Jelly Matrix Decellularization for Tissue Engineering Applications.- 3D-Decellularized Native Extra Cellular Matrix Scaffold for In Vitro Culture Expansion of Human Wharton Jelly Mesenchymal Stem Cells (hWJ MSCs).- Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells.- Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells.- Mouse Skeletal Muscle Decellularization.- Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering.- In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering.- Glutaraldehyde Crosslinking Modification of Decellularized Rat Kidney Scaffolds.- Preparation of Scaffolds from Decellularized Testicular Matrix.- Decellularization of Bovine Small Intestinal Submucosa.- Decellularization and Recellularization of Cartilage.- A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration.- Tracheal Cartilage Isolation and Decellularization.- Protocols for Rat Uterus Isolation and Decellularization: Applications for Uterus Tissue Engineering and 3D Cell Culturing.- Application of Decellularized Tissue Scaffolds in Ovarian Tissue Transplantation.- Composite Bioscaffolds Incorporating Decellularized ECM as a Cell-Instructive Component within Hydrogels as In Vitro Models and Cell Delivery Systems.- 3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications.- Decellularization of Large Tendon Specimens: Combination of Manually Performed Freeze Thaw Cycles and Detergent Treatment.- Decellularized Bone Matrix Scaffold for Bone Regeneration.- Preparation of Decellularized Liver Scaffolds and Recellularized Liver Grafts.- Decellularization of Liver and Organogenesis in Rats.- Simple and Quick Method to Obtain a Decellularized, Functional Liver Bioscaffold.- Liver Bioengineering Using Decellularized Whole-Liver Scaffolds.- Decellularization of Intact Lung Tissue through Vasculature and Airways Using Negative and Positive Pressure.- Alveolar Rhabdomyosarcoma Decellularization.
Decellularization Methods for Scaffold Fabrication.- Decellularized Liver Scaffold for Liver Regeneration.- Wharton's Jelly Matrix Decellularization for Tissue Engineering Applications.- 3D-Decellularized Native Extra Cellular Matrix Scaffold for In Vitro Culture Expansion of Human Wharton Jelly Mesenchymal Stem Cells (hWJ MSCs).- Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells.- Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells.- Mouse Skeletal Muscle Decellularization.- Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering.- In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering.- Glutaraldehyde Crosslinking Modification of Decellularized Rat Kidney Scaffolds.- Preparation of Scaffolds from Decellularized Testicular Matrix.- Decellularization of Bovine Small Intestinal Submucosa.- Decellularization and Recellularization of Cartilage.- A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration.- Tracheal Cartilage Isolation and Decellularization.- Protocols for Rat Uterus Isolation and Decellularization: Applications for Uterus Tissue Engineering and 3D Cell Culturing.- Application of Decellularized Tissue Scaffolds in Ovarian Tissue Transplantation.- Composite Bioscaffolds Incorporating Decellularized ECM as a Cell-Instructive Component within Hydrogels as In Vitro Models and Cell Delivery Systems.- 3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications.- Decellularization of Large Tendon Specimens: Combination of Manually Performed Freeze Thaw Cycles and Detergent Treatment.- Decellularized Bone Matrix Scaffold for Bone Regeneration.- Preparation of Decellularized Liver Scaffolds and Recellularized Liver Grafts.- Decellularization of Liver and Organogenesis in Rats.- Simple and Quick Method to Obtain a Decellularized, Functional Liver Bioscaffold.- Liver Bioengineering Using Decellularized Whole-Liver Scaffolds.- Decellularization of Intact Lung Tissue through Vasculature and Airways Using Negative and Positive Pressure.- Alveolar Rhabdomyosarcoma Decellularization.
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