The papers assembled in this volume are based on the symposium on "The Biochemistry of Gene Expression in Higher Organisms" which was held at the University of Sydney from May 14-19, 1972. Many symposia have been held on the control of gene expression in prokaryotes but to date considerably less attention has been paid to eukaryotic organisms. It has been appreciated only recently that some of the information gained from the study of prokaryotes is directly applicable to eukaryotes; however, it is now realized that the principles of the control mechanisms of gene expression in these two…mehr
The papers assembled in this volume are based on the symposium on "The Biochemistry of Gene Expression in Higher Organisms" which was held at the University of Sydney from May 14-19, 1972. Many symposia have been held on the control of gene expression in prokaryotes but to date considerably less attention has been paid to eukaryotic organisms. It has been appreciated only recently that some of the information gained from the study of prokaryotes is directly applicable to eukaryotes; however, it is now realized that the principles of the control mechanisms of gene expression in these two classes of organism, differ considerably. This symposium was organized in an effort to bring together workers from widely different fields concerned with gene expression, with the aim of circum scribing the current concepts and speculating on future developments in studies on the mechanisms which control and modulate gene expression, in the widest sense, in eukaryotes. This volume contains all the36 papers presented at the symposium. In a few instances the sequence of contributions has been changed to provide the reader with a more logical presentation. In addition, three papers which were not actually presented at the symposium, have been included in this volume. These three papers were not read because last-minute hitches prevented speakers from attending.
Chromosome Structure and the Manipulation and Analysis of Genes.- Chromosome structure and units of function in higher organisms.- Transgenosis of bacterial genes from Escherichia coli to cultures of haploid Lycopersicon esculentum and haploid Arabidopsis thaliana plant-cells.- Sequences in genetic nucleic acids.- Mutations at the end of the iso-l-cytochrome c gene of yeast.- Transcriptional and Translational Control Mechanisms.- RNA polymerases and transcriptive specificity in eukaryotic organisms.- The control of gene expression by membrane organization in Saccharoinyces Cerevisiae.- Transcription and translation in mammalian cells.- Isolated chromatin in the study of gene expression.- Structural modifications of histones in cultured mammalian cells.- The role of histones in avian erythropoiesis.- Chromosomal components in relation to differentiation of avian red blood cells.- Mechanism of glucocorticoid hormone action and of regulation of gene expression in cultured mammalian cells.- Regulation of transcription by glucocorticosteroids.- A control mechanism in gene expression of higher cells operating at the termination step in protein synthesis.- The mechanism and control of the biosynthesis of ?-lactalbumin by the mammary gland.- Changes in protein synthesis and degradation involved in enzyme accumulation in differentiating liver.- Gene Expression and Development.- Obligatory requirement for DNA synthesis during myogenesis erythrogenesis and chondrogenesis.- The dependence of gene expression on membrane assembly.- Patterns of gene activity in larval tissues of the blowfly Calliphora.- Hormonal and environmental modulation of gene expression in plant development.- DNA and RNA synthesis during growth by cell expansion in Vicia faba cotyledons.- Gene Expression in Differentiated Cells.- Induction of ?-aminolevulinic acid synthetase in perfused rat liver by drugs, steroids, lead and adenosine-3?, 5? -monophosphate.- The anaemia-induced reversible switch from haemoglobin A to haemoglobin C in goats and sheep: the two haemoglobins are present in the same cell during the changeover.- Gene expression in liver endoplasmic reticulum.- The use of neurological mutants as experimental models.- Gene Expression in Mitochondria and Chloroplasts.- The phenomenology of cytoplasmic genetics in yeast: a proposal for an autonomy of mitochondrial membranes and the determinism of nucleo-cytoplasmic genetic interactions.- Location of DNAs coding for various kinds of chloroplast proteins.- Nuclear genes controlling chloroplast development in barley.- Gene expression in chloroplasts and regulation of chloroplast differentiation.- Products of chloroplast DNA-directed transcription and translation.- Gene Expression and the Immune Response.- The relevance of immunology to the biochemistry of gene expression.- The reaction of antigen with lymphocytes.- Synthesis transport and secretion of immunoglobulin in lymphoid cells.- Molecular and cellular mechanisms of clonal selection.- Antibody diversification: the somatic mutation model revisited.- An alternate mechanism for immune recognition.- The clonal development of antibody forming cells.- Immunoglobulin gene expression in murine lymphoid cells.- Structure and function of lymphocyte surface immunoglobulin.- List of Abbreviations.
Chromosome Structure and the Manipulation and Analysis of Genes.- Chromosome structure and units of function in higher organisms.- Transgenosis of bacterial genes from Escherichia coli to cultures of haploid Lycopersicon esculentum and haploid Arabidopsis thaliana plant-cells.- Sequences in genetic nucleic acids.- Mutations at the end of the iso-l-cytochrome c gene of yeast.- Transcriptional and Translational Control Mechanisms.- RNA polymerases and transcriptive specificity in eukaryotic organisms.- The control of gene expression by membrane organization in Saccharoinyces Cerevisiae.- Transcription and translation in mammalian cells.- Isolated chromatin in the study of gene expression.- Structural modifications of histones in cultured mammalian cells.- The role of histones in avian erythropoiesis.- Chromosomal components in relation to differentiation of avian red blood cells.- Mechanism of glucocorticoid hormone action and of regulation of gene expression in cultured mammalian cells.- Regulation of transcription by glucocorticosteroids.- A control mechanism in gene expression of higher cells operating at the termination step in protein synthesis.- The mechanism and control of the biosynthesis of ?-lactalbumin by the mammary gland.- Changes in protein synthesis and degradation involved in enzyme accumulation in differentiating liver.- Gene Expression and Development.- Obligatory requirement for DNA synthesis during myogenesis erythrogenesis and chondrogenesis.- The dependence of gene expression on membrane assembly.- Patterns of gene activity in larval tissues of the blowfly Calliphora.- Hormonal and environmental modulation of gene expression in plant development.- DNA and RNA synthesis during growth by cell expansion in Vicia faba cotyledons.- Gene Expression in Differentiated Cells.- Induction of ?-aminolevulinic acid synthetase in perfused rat liver by drugs, steroids, lead and adenosine-3?, 5? -monophosphate.- The anaemia-induced reversible switch from haemoglobin A to haemoglobin C in goats and sheep: the two haemoglobins are present in the same cell during the changeover.- Gene expression in liver endoplasmic reticulum.- The use of neurological mutants as experimental models.- Gene Expression in Mitochondria and Chloroplasts.- The phenomenology of cytoplasmic genetics in yeast: a proposal for an autonomy of mitochondrial membranes and the determinism of nucleo-cytoplasmic genetic interactions.- Location of DNAs coding for various kinds of chloroplast proteins.- Nuclear genes controlling chloroplast development in barley.- Gene expression in chloroplasts and regulation of chloroplast differentiation.- Products of chloroplast DNA-directed transcription and translation.- Gene Expression and the Immune Response.- The relevance of immunology to the biochemistry of gene expression.- The reaction of antigen with lymphocytes.- Synthesis transport and secretion of immunoglobulin in lymphoid cells.- Molecular and cellular mechanisms of clonal selection.- Antibody diversification: the somatic mutation model revisited.- An alternate mechanism for immune recognition.- The clonal development of antibody forming cells.- Immunoglobulin gene expression in murine lymphoid cells.- Structure and function of lymphocyte surface immunoglobulin.- List of Abbreviations.
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