In recent years there has been a growing awareness of the importance of reproductive biology to crop production and there has been a tremendous increase in research on reproductive structures of higher plants. Presented here is a wide information of different aspects of micro- and macrosporogenesis, pollen-stigma interaction and recognition, pollen tube growth, cytoskeleton, in vitro and in vivo gamete fusion, and incompatibility. The most advanced techniques employed in studies on reproductive biology of higher plants are described in detail.
In recent years there has been a growing awareness of the importance of reproductive biology to crop production and there has been a tremendous increase in research on reproductive structures of higher plants. Presented here is a wide information of different aspects of micro- and macrosporogenesis, pollen-stigma interaction and recognition, pollen tube growth, cytoskeleton, in vitro and in vivo gamete fusion, and incompatibility. The most advanced techniques employed in studies on reproductive biology of higher plants are described in detail.
Microspore derived embryogenesis.- Gene expression during microsporogenesis.- Gene expression during pollen development.- Cytological techniques to assess pollen quality.- Megasporogenesis and megagametogenesis.- The angiosperm stigma.- Transport mechanisms of pollen - a short review.- Pollen capture, adhesion and hydration.- Pollen tube emission, organization and tip growth.- The cytoskeleton of pollen grains and pollen tubes.- Sporophytic and gametophytic self-incompatibility.- Application of in vitro pollination and fertilization techniques for breeding and genetic manipulation of Lilium.- Pollen tube entrance in the embryo sac and fertilization.- Preferential fertilization: data and strategy for molecular analysis.- Effects of gametophytic selection on the genetic structure of populations.- The isolation of sperm cells, their microinjection into the egg apparatus and methods for structural analysis of the injected cells.- Micromanipulation and in vitro fertilization with single pollen grains and isolated gametes of maize.- In situ and in vitro aspects of embryo-ovule interactions in intra - and interspecific beet crosses.- Stress effects on the male gametophyte.- Mature pollen and its impact on plant and man.- Pollen for monitoring environmental pollution.- Sound propagation in the natural environment, animal acoustic communication and possible impact for pollination.- Confocal laser scanning microscopy and its applications in research on sexual plant reproduction.- Author index.
Microspore derived embryogenesis.- Gene expression during microsporogenesis.- Gene expression during pollen development.- Cytological techniques to assess pollen quality.- Megasporogenesis and megagametogenesis.- The angiosperm stigma.- Transport mechanisms of pollen - a short review.- Pollen capture, adhesion and hydration.- Pollen tube emission, organization and tip growth.- The cytoskeleton of pollen grains and pollen tubes.- Sporophytic and gametophytic self-incompatibility.- Application of in vitro pollination and fertilization techniques for breeding and genetic manipulation of Lilium.- Pollen tube entrance in the embryo sac and fertilization.- Preferential fertilization: data and strategy for molecular analysis.- Effects of gametophytic selection on the genetic structure of populations.- The isolation of sperm cells, their microinjection into the egg apparatus and methods for structural analysis of the injected cells.- Micromanipulation and in vitro fertilization with single pollen grains and isolated gametes of maize.- In situ and in vitro aspects of embryo-ovule interactions in intra - and interspecific beet crosses.- Stress effects on the male gametophyte.- Mature pollen and its impact on plant and man.- Pollen for monitoring environmental pollution.- Sound propagation in the natural environment, animal acoustic communication and possible impact for pollination.- Confocal laser scanning microscopy and its applications in research on sexual plant reproduction.- Author index.
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