Antigen Retrieval Immunohistochemistry Based Research and Diagnostics (eBook, PDF)

Redaktion: Shi, Shan-Rong; Taylor, Clive R.

• Format: PDF

Produktbeschreibung

The most complete, up-to-date reference on antigen retrieval and immunohistochemistry An antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment. Antigen Retrieval Immunohistochemistry Based Research and Diagnostics discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and application of AR by the editors, this volume summarizes recent achievements in AR-IHC and analyzes numerous cutting-edge issues for future research projects. Featuring contributions from a worldwide group of leading experts and research scientists in the field, this important work: * Summarizes the key problems in the four fields of antigen retrieval * Discusses the advances of AR techniques and their applications * Provides practical methods and protocols in AR-IHC, such as extraction of nucleic acids and proteins for molecular analysis, cell/tissue sample preparation, and standardization and development of various techniques to meet the future needs of¿clinical and research molecular analysis * Encourages further research in AR and IHC, particularly how AR methods might be employed for improved test performance and the development of greater reliability and reproducibility of IHC * Includes an appendix of related laboratory protocols Antigen Retrieval Immunohistochemistry Based Research and Diagnostics is intended for clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students who undertake tissue/cell morphologic and molecular analysis and wish to use and extend the power of immunohistochemistry. It is also pertinent for most biotechnology companies majoring in development of IHC products. Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems / Kai Chang, Series Editor

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 464
• Erscheinungstermin: 20. Juli 2010
• Englisch
• ISBN-13: 9780470875605
• Artikelnr.: 37299810

Autorenporträt

Shan-Rong Shi, MD, is professor of clinical pathology at the Keck School of Medicine of the University of Southern California. He is the Associate Editor of The Journal of Histochemistry and cytochemistry, he is on the Editoral Board of Applied Immunohistochemistry and Molecular Morphology and is also on the Overseas Editorial Board of the Chinese Journal of Pathology. Dr. Shi is the ineventor of the antigen retrieval (AR) technique. Clive R. Taylor, MD, PhD, is Senior Associate Dean, Professor and Chairman of Pathology at the Keck School of Medicine. Dr. Taylor is Editor in Chief of Applied Immunohistochemistry and Molecular Morphology. Dr. Taylor is a world renowned expert in immunohistochemistry and clinical pathology.

Inhaltsangabe

PREFACE. CONTRIBUTORS. PART I: RECENT ADVANCES IN ANTIGEN RETRIEVAL
TECHNIQUES AND ITS APPLICATION. 1 STANDARDIZATION OF ANTIGEN RETRIEVAL
TECHNIQUES BASED ON THE TEST BATTERY APPROACH (Shan-Rong Shi and Clive R.
Taylor). 2 EXTENDED APPLICATION OF ANTIGEN RETRIEVAL TECHNIQUE IN
IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION (Shan-Rong Shi and Clive R.
Taylor). 3 EXTRACTION OF DNA/RNA FROM FORMALIN-FIXED, PARAFFIN-EMBEDDED
TISSUE BASED ON THE ANTIGEN RETRIEVAL PRINCIPLE (Shan-Rong Shi and Clive R.
Taylor). PART II: STANDARDIZATION OF IMMUNOHISTOCHEMISTRY. 4 KEY ISSUES AND
STRATEGIES OF STANDARDIZATION FOR QUANTIFIABLE IMMUNOHISTOCHEMISTRY
(Shan-Rong Shi, Kevin A. Roth, and Clive R. Taylor). 5 STANDARDIZATION OF
IMMUNOHISTOCHEMISTRY BASED ON ANTIGEN RETRIEVAL TECHNIQUE (Shan-Rong Shi
and Clive R. Taylor). 6 STANDARD REFERENCE MATERIAL: CELL LINE DEVELOPMENT
AND USE OF REFERENCE CELL LINES AS STANDARDS FOR EXTERNAL QUALITY ASSURANCE
OF HER2 IHC AND ISH TESTING (Bharat Jasani, Vicky Reid, Colin Tristram,
Jeremy Walker, Paul Scorer, Michael Morgan, John Bartlett, Merdol Ibrahim,
and Keith Miller). 7 PEPTIDES AS IMMUNOHISTOCHEMISTRY CONTROLS (Steven A.
Bogen and Seshi R. Sompuram). 8 STANDARD REFERENCE MATERIAL:
PROTEINEMBEDDING TECHNIQUE AND DESIGN OF BAR CODE (Shan-Rong Shi, Jiang Gu,
and Clive R. Taylor). 9 THE PROS AND CONS OF AUTOMATION FOR
IMMUNOHISTOCHEMISTRY FROM THE PROSPECTIVE OF THE PATHOLOGY LABORATORY
(David G. Hicks and Loralee McMahon). 10 IMAGE ANALYSIS IN
IMMUNOHISTOCHEMISTRY (Alton D. Floyd). PART III TISSUE/CELL SAMPLE
PREPARATION. 11 TISSUE CELL SAMPLE PREPARATION: LESSONS FROM THE ANTIGEN
RETRIEVAL TECHNIQUE (Shan-Rong Shi and Clive R. Taylor). 12 MECHANISMS OF
ACTION AND PROPER USE OF COMMON FIXATIVES (Richard W. Dapson). 13 CELL
SAMPLE PREPARATION FOR CLINICAL CYTOPATHOLOGY: CURRENT STATUS AND FUTURE
DEVELOPMENT (Yan Shi and Patricia G. Wasserman). 14 DESIGN OF A TISSUE
SURROGATE TO EXAMINE ACCURACY OF PROTEOMIC ANALYSIS (Carol B. Fowler,
Jeffrey T. Mason, and Timothy J. O'leary). PART IV: MOLECULAR MECHANISM OF
ANTIGEN RETRIEVAL TECHNIQUE. 15 STUDY OF FORMALIN FIXATION AND HEAT-INDUCED
ANTIGEN RETRIEVAL (Jeffrey T. Mason, Carol B. Fowler, and Timothy J.
O'leary). 16 A LINEAR EPITOPES MODEL OF ANTIGEN RETRIEVAL (Steven A. Bogen
and Seshi R. Sompuram). 17 PH OR IONIC STRENGTH OF ANTIGEN RETRIEVAL
SOLUTION: A POTENTIAL ROLE FOR REFOLDING DURING HEAT TREATMENT (Shuji
Yamashita). 18 COMMENTARY: FUTURE DIRECTIONS (Timothy J. O'Leary, Carol B.
Fowler, David L. Evers, Robert E. Cunningham, and Jeffrey T. Mason). PART
V: PROTEOMIC ANALYSIS OF PROTEIN EXTRACTED FROM TISSUE/CELLS. 19 TECHNIQUES
OF PROTEIN EXTRACTION FROM FFPE TISSUE/CELLS FOR MASS SPECTROMETRY (Carol
B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason). 20 APPLICATION OF
SHOTGUN PROTEOMICS TO FORMALIN-FIXED AND PARAFFIN-EMBEDDED TISSUES (Brian
M. Balgley). 21 VISUALIZING PROTEIN MAPS IN TISSUE (Masahiro Mukai and
Mitsutoshi Setou). 22 SYMBIOSIS OF IMMUNOHISTOCHEMISTRY AND PROTEOMICS:
MARCHING TO A NEW ERA (Shan-Rong Shi, Brian M. Balgley, and Clive R.
Taylor). APPENDIX RELATED LABORATORY PROTOCOLS. INDEX.