Microinjection
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This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in…mehr

Produktbeschreibung
This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations.
  • Produktdetails
  • Methods in Molecular Biology .1874
  • Verlag: Humana Press / Springer New York / Springer, Berlin
  • Artikelnr. des Verlages: 978-1-4939-8830-3
  • 1st ed. 2019
  • Seitenzahl: 556
  • Erscheinungstermin: 24. Oktober 2018
  • Englisch
  • Abmessung: 260mm x 183mm x 35mm
  • Gewicht: 1196g
  • ISBN-13: 9781493988303
  • ISBN-10: 1493988301
  • Artikelnr.: 53039563
Inhaltsangabe
1. Microinjection and Micromanipulation: A Historical Perspective

Wenhao Xu

2. Production of Transgenic Mice by Pronuclear Microinjection

Xin-an Pu, Anthony P. Young, and H. Michael Kubisch

3. Transgene Recombineering in Bacterial Artificial Chromosomes

Michael G. Zeidler and Thomas L. Saunders

4. Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice

Ruby Yanru Chen-Tsai

5. Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method

Takuya Sato and Takehiko Ogawa

6. Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer

Yubin Du, Wen Xie, Fan Zhang, and Chengyu Liu

7. Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection

Pinghu Liu, Yan Li, Jingqi Lei, and Lijin Dong

8. Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice

Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, and Colin L. Sweeney

9. Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique

Melissa A. Scott and Yueh-Chiang Hu

10. Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation

Wenning Qin and Haoyi Wang

11. Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides

Ye Liu, Yubin Du, Wen Xie, Fan Zhang, Douglas Forrest, and Chengyu Liu

12. Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection

Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama

13. The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications

Naomi Nakagata, Jorge Sztein, and Toru Takeo

14. The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm

Toru Takeo, Jorge Sztein, and Naomi Nakagata

15. Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line

Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, and Takayuki Shindo

16. Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology

Tuija M. Alcantar and Xin Y. Rairdan

17. Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos

Xiaoxia Cui

18. Organ Generation from Knocked-Out Rat Blastocysts Complemented with Pluripotent Stem Cells

Masumi Hirabayashi and Shinichi Hochi

19. Generation of Rabbit Models by Gene Editing Nucleases

Dongshan Yang, Jie Xu, and Y. Eugene Chen

20. Production of Genetically Engineered Porcine Embryos by Handmade Cloning

Rong Li, Jinxin Miao, and Zhongde Wang

21. Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production

Qingran Kong and Zhonghua Liu

22. Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer

Zhiqiang Fan, Min Yang, Misha Regouski, and Irina A. Polejaeva

23. Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting

Liyou An, Lan Yang, Yuejin Huang, Yong Cheng, and Fuliang Du

24. Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes

Ellen J. Collarini, Philip A. Leighton, and Marie-Cecile van de Lavoir

25. Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing

Jyoti Iyer, Nicole DeVaul, Tyler Hansen, and Ben Nebenfuehr

26. Microinjection in Zebrafish for Genome Editing and Functional Studies

Wuhong Pei and Shawn M. Burgess

27. Microinjection of Marine Fish Eggs

Rie Goto, Taiju Saito, Takahiro Matsubara, and Etsuro Yamaha

28. Generating Gene Knockout Oryzias Latipes and Rice Field Eel Using TALENs Method

Daji Luo, Ke Feng, Zuoyan Zhu, and Wei Hu

29. Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus

Yuki Shibata, Lingyu Bao, Liezhen Fu, Bingyin Shi, and Yun-Bo Shi

30. Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function

Robert F. Gahl