Synthetic Biology and Metabolic Engineering in Plants and Microbes: Part A, the new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods, synthetic biology, and metabolic engineering in plants and microbes, and includes sections on such topics as the uses of integrases in microbial engineering, biosynthesis, and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways.
Synthetic Biology and Metabolic Engineering in Plants and Microbes: Part A, the new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.
This volume covers research methods, synthetic biology, and metabolic engineering in plants and microbes, and includes sections on such topics as the uses of integrases in microbial engineering, biosynthesis, and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways.
1. Directing Biosynthesis: Practical Supply of Natural and Unnatural Cyanobactins Debosmita Sardar, Ma Diarey Tianero and Eric W. Schmidt 2. Synthetic Biology Approaches to New Bisindoles Lona M. Alkhalaf, Yi-Ling Du and Katherine S. Ryan 3. Enzymatic [4+2] Cycloadditions in the Biosynthesis of Spirotetramates and Spirotetronates Bo Pang, Guannan Zhong, Zhijun Tang and Wen Liu 4. Application and Modification of Flavin-Dependent Halogenases Karl-Heinz van Pée, Daniela Milbredt, Eugenio Patallo, Veit Weichold and Maria Gajewi 5. Engineering Flavin-Dependent Halogenases James T. Payne, Mary C. Andorfer and Jared C. Lewis 6. Heterologous Expression of Fungal Secondary Metabolite Pathways in the Aspergillus Nidulans Host System Johannes W. A. van Dijk and Clay C. C. Wang 7. Plug-and-Play Benzylisoquinoline Alkaloid Biosynthetic Gene Discovery in Engineered Yeast Jeremy S. Morris, Mehran Dastmalchi, Jing Li, Limei Chang, Xue Chen, Jillian M. Hagel and Peter J. Facchini 8. Optimizing Metabolic Pathways for the Improved Production of Natural Products J. Andrew Jones and Mattheos A. G. Koffas 9. Reconstituting Plant Secondary Metabolism in Saccharomyces Cerevisiae for Production of High-Value Benzylisoquinoline Alkaloids Michael E. Pyne, Lauren Narcross, Elena Fossati, Leanne Bourgeois, Euan Burton, Nicholas D. Gold and Vincent J.J. Martin 10. Engineering Microbes to Synthesize Plant Isoprenoids Kang Zhou, Steven Edgar and Gregory Stephanopoulos 11. Natural Product Biosynthesis in Escherichia Coli: Mentha Monoterpenoids Helen S. Toogood, Shirley Tait, Adrian Jervis, Aisling Ní Cheallaigh, Luke Humphreys, Eriko Takano, John M. Gardiner and Nigel S. Scrutton 12. High-Efficiency Genome Editing of Streptomyces Species by an Engineered Crispr/Cas System Yajie Wang, Ryan E. Cobb and Huimin Zhao 13. Rapid optimization of engineered metabolic pathways with Serine Integrase Recombinational Assembly (SIRA) Christine Merrick, Caroline Wardrope, Jane Paget, Sean Colloms and Susan Rosser 14. Rewiring Riboswitches to Create New Genetic Circuits in Bacteria Christopher J. Robinson, Daniel Medina-Stacey, Ming-Cheng Wu, Helen A. Vincent and Jason Micklefield
1. Directing Biosynthesis: Practical Supply of Natural and Unnatural Cyanobactins Debosmita Sardar, Ma Diarey Tianero and Eric W. Schmidt 2. Synthetic Biology Approaches to New Bisindoles Lona M. Alkhalaf, Yi-Ling Du and Katherine S. Ryan 3. Enzymatic [4+2] Cycloadditions in the Biosynthesis of Spirotetramates and Spirotetronates Bo Pang, Guannan Zhong, Zhijun Tang and Wen Liu 4. Application and Modification of Flavin-Dependent Halogenases Karl-Heinz van Pée, Daniela Milbredt, Eugenio Patallo, Veit Weichold and Maria Gajewi 5. Engineering Flavin-Dependent Halogenases James T. Payne, Mary C. Andorfer and Jared C. Lewis 6. Heterologous Expression of Fungal Secondary Metabolite Pathways in the Aspergillus Nidulans Host System Johannes W. A. van Dijk and Clay C. C. Wang 7. Plug-and-Play Benzylisoquinoline Alkaloid Biosynthetic Gene Discovery in Engineered Yeast Jeremy S. Morris, Mehran Dastmalchi, Jing Li, Limei Chang, Xue Chen, Jillian M. Hagel and Peter J. Facchini 8. Optimizing Metabolic Pathways for the Improved Production of Natural Products J. Andrew Jones and Mattheos A. G. Koffas 9. Reconstituting Plant Secondary Metabolism in Saccharomyces Cerevisiae for Production of High-Value Benzylisoquinoline Alkaloids Michael E. Pyne, Lauren Narcross, Elena Fossati, Leanne Bourgeois, Euan Burton, Nicholas D. Gold and Vincent J.J. Martin 10. Engineering Microbes to Synthesize Plant Isoprenoids Kang Zhou, Steven Edgar and Gregory Stephanopoulos 11. Natural Product Biosynthesis in Escherichia Coli: Mentha Monoterpenoids Helen S. Toogood, Shirley Tait, Adrian Jervis, Aisling Ní Cheallaigh, Luke Humphreys, Eriko Takano, John M. Gardiner and Nigel S. Scrutton 12. High-Efficiency Genome Editing of Streptomyces Species by an Engineered Crispr/Cas System Yajie Wang, Ryan E. Cobb and Huimin Zhao 13. Rapid optimization of engineered metabolic pathways with Serine Integrase Recombinational Assembly (SIRA) Christine Merrick, Caroline Wardrope, Jane Paget, Sean Colloms and Susan Rosser 14. Rewiring Riboswitches to Create New Genetic Circuits in Bacteria Christopher J. Robinson, Daniel Medina-Stacey, Ming-Cheng Wu, Helen A. Vincent and Jason Micklefield
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