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From the present study, glyphosate induced biochemical, histopathological, and subcellular alterations in hepatocytes of liver structure in male and female rats. Results from this investigation may contribute to the understanding of the effect(s) of different doses and simultaneous exposition to glyphosate on cell survival, and they may be of clinical interest in the evaluation of the degree of damage to which humans are involuntarily exposed due to environmental pollution.we should to be very careful during use of glyphosate as an herbicide in our fields and that we should use it in a specific dose according to WHO to reduce as much as possible its passive effects. We should also overcome the problems resulting from eating food may contain glyphosate. Taking a good antioxidant to strengthen our immune system can provide one of the possible remedies.