Henry Erlich

PCR Technology (eBook, PDF)

Principles and Applications for DNA Amplification

• Format: PDF

Produktbeschreibung

This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.

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Produktdetails

• Verlag: Springer-Verlag GmbH
• Seitenzahl: 246
• Erscheinungstermin: 31. Dezember 2015
• Englisch
• ISBN-13: 9781349202355
• Artikelnr.: 45968534

Inhaltsangabe

Part 1 Introduction to the PCR: history of PCR development; description of the PCR method - basic principles and properties of the PCR; thermostable DNA polymerases; automation; sample preparation. Part 2 Research applications - brief overview: direct sequencing using PCR; preparation of specific DNA fragments using PCR; detection of gene expression; genetic mapping using PCR amplification of individual gametes. Part 3 Diagnostic applications - brief overview: detection of pathogens; diagnosis of monogenic genetic diseases; HLA DNA typing and disease susceptibility; forensic applications; detection of activated oncogenes; future developments and applications.