Biosensors and Their Applications - Yang, Victor C. / Ngo, That T. (Hgg.)
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A biosensor is a device in which a bioactive layer lies in direct contact with a transducer whose responses to change in the bioactive layer generate eloctronic signals for interpretation. The bioactive layer may consist of membrane-bound enzymes, anti-bodies, or receptors. The potential of this blend of electronics and biotechnology includes the direct assay of clinically important substrates (e.g. blood glucose) and of substances too unstable for storage or whose concentrations fluctuate rapidly. Written by the leading researchers in the field, this book reflects the most current…mehr

Produktbeschreibung
A biosensor is a device in which a bioactive layer lies in direct contact with a transducer whose responses to change in the bioactive layer generate eloctronic signals for interpretation. The bioactive layer may consist of membrane-bound enzymes, anti-bodies, or receptors. The potential of this blend of electronics and biotechnology includes the direct assay of clinically important substrates (e.g. blood glucose) and of substances too unstable for storage or whose concentrations fluctuate rapidly. Written by the leading researchers in the field, this book reflects the most current developments in successfully constructing a biosensor. Major applications are in the fields of pharmacology, molecular biology, virology and electronics.
  • Produktdetails
  • Verlag: Springer, Berlin
  • 2000.
  • Seitenzahl: 388
  • Erscheinungstermin: 30. April 2000
  • Englisch
  • Abmessung: 260mm x 183mm x 25mm
  • Gewicht: 953g
  • ISBN-13: 9780306460876
  • ISBN-10: 0306460874
  • Artikelnr.: 22108109
Inhaltsangabe
1. Biochromic Polydiacetylene Synthetic Membranes.- 1.1. Introduction.- 1.2 Assembling the System.- 1.3 Membranelike Structures in Biosensing.- 1.4 Sensors and Biosensors Based on Conjugated Polymers.- 1.4.1. Basic Properties.- 1.4.2. Conjugated Polymer-Based Sensors.- 1.4.3. Polydiacetylenes and Chromic Effects.- 1.4.4. PDA-Based Biosensors.- 1.5 Conclusion.- References.- 2. Analysis of the Kinetics of Antigen-Antibody Interactions and Fractal Dimension in Biosensors.- 2.1. Introduction.- 2.2. Theory.- 2.2.1. Single-Fractal Analysis.- 2.2.2. Dual-Fractal Analysis.- 2.3. Results.- 2.4. Conclusions.- References.- 3. Avidin-Biotin Mediated Biosensors.- 3.1. Introduction.- 3.2. Avidin-Biotin System.- 3.3. Immobilization of Enzymes Through Avidin-Biotin Complexation.- 3.4. Layer-by-Layer Structure of Enzyme Multilayers.- 3.5. Conclusions.- References.- 4. Layered Functionalized Electrodes for Electrochemical Biosensor Applications.- 4.1. Introduction.- 4.2. Monolayer Enzyme Electrodes.- 4.3. Electrical Contact of Monolayer and Multilayer Enzyme Electrodes.- 4.4. Electrically Contacted Reconstituted Enzyme Electrodes.- 4.5. Integrated Layered NAD(P)+-Dependent Enzyme Electrodes.- 4.6. Layered Antigen Monolayer Electrodes for Electrochemical Probing of Antigen-Antibody Interactions.- 4.7. Layered Photoisomerizable Antigen Monolayer Electrodes for Reversible Probing of Antigen-Antibody Interactions.- 4.8. Layered Oligonucleotide Electrodes for Electrochemical Probing of DNA.- 4.9. Conclusions and Perspectives.- References.- 5. Biosensors Based on "Wired" Peroxidases.- 5.1. Introduction.- 5.2. "Wiring" of Horseradish Peroxidase.- 5.3. Thermostable Soybean Peroxidase.- 5.4. Bienzyme Systems.- 5.5. Applications.- 5.5.1. NAD(P)H Sensing.- 5.5.2. Avidin and Biotin.- 5.5.3. Oligonucleotide Sensing.- 5.5.4. Characterization of Electrodes Generating and Consuming H2O2.- 5.5.5. Organic-Phase Peroxide Sensors.- References.- 6. Nonseparation Electrochemical Enzyme Immunoassay Using Microporous Gold Electrodes.- 6.1. Introduction.- 6.2. Experimental.- 6.2.1. Apparatus.- 6.2.2. Reagents.- 6.2.3. Preparation of Microporous Gold Electrodes and Immobilization of Binding Proteins.- 6.2.4. Nonseparation Sandwich-Type Electrochemical Enzyme Immunoassays.- 6.2.5. Nonseparation Competitive Electrochemical Enzyme Binding/Immunoassay.- 6.3. Results and Discussion.- 6.3.1. Noncompetitive Electrochemical Enzyme Immunoassay for Proteins and Microorganisms.- 6.3.2. Competitive Electrochemical Enzyme Binding Assay for Small Molecules.- 6.4. Future Directions.- 6.5. Conclusions.- References.- 7. Liposomes as Signal-Enhancement Agents in Immunodiagnostic Applications.- 7.1. Introduction.- 7.2. Amplification of an Enzyme Immunoassay Using Liposomes.- 7.2.1. Preparation of Enzyme-and Antibody-Bearing Liposomes.- 7.2.2. Preparation of Enzyme-Antibody Conjugate.- 7.2.3. Characterization of Liposomes with Immobilized HRP and Antibody.- 7.2.4. Sandwich ELISA with Liposomes and Enzyme-Antibody Conjugate.- 7.2.5. Results and Discussion.- 7.3. Amplification of Fluoroimmunoassay Using Liposomes.- 7.3.1. Preparation of Antibody-Bearing Fluorescent Liposomes.- 7.3.2. Preparation of Fluor-Antibody Conjugate.- 7.3.3. Fluoroimmunoassay with Liposomes and Fluorescein-Antibody Conjugate.- 7.3.4. Results and Discussion.- 7.4. Application of Ganglioside-Bearing Liposomes as Sensitive Probes for Potent Neurotoxins.- 7.4.1. Preparation and Characterization of GT1b Liposomes.- 7.4.2. Fluoroimmunoassay with GT1b Liposomes.- 7.4.3. Results and Discussion.- 7.5. Conclusions.- References.- 8. Recent Development in Polymer Membrane-Based Potentiometric Polyion Sensors.- 8.1. Introduction.- 8.2. Development of Polymer Membrane-Based Polyion Sensors.- 8.2.1. Extraction Chemistry.- 8.2.2. Response Slope.- 8.3. Applications of Polyion Sensors.- 8.3.1. Measuring the Blood Heparin Levels.- 8.3.2. Probing Binding Reactions.- 8.3.3. Detecting Protease Activities.- 8.4. Conclusions.- References