Marvin Mcmaster

HPLC (eBook, PDF)

A Practical User's Guide

• Format: PDF

Produktbeschreibung

This Second Edition of the classic handbook details how to set up an HPLC system that capitalizes on the latest innovations. It covers new techniques in high-temperature, micro-flow, and ultra-fast chromatography, the linking of an HPLC to a mass spectrometer, and more. Complete appendices and supplementary material online, this guide has everything chromatographers need to know to confidently separate, identify, purify, and quantify compounds.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 256
• Erscheinungstermin: 4. Januar 2007
• Englisch
• ISBN-13: 9780470079089
• Artikelnr.: 37290014

Autorenporträt

MARVIN C. MCMASTER, PHD, is a consultant as well as an Adjunct Professor of Chemistry at the University of Missouri-- St. Louis. Dr. McMaster has served as a researcher and product developer for DuPont, Kraft Foods, and Ciba-Geigy. Among his other publications, Dr. McMaster is author of LC/MS: A Practical User's Guide and coauthor of GC/MS: A Practical User's Guide (both published by Wiley).

Inhaltsangabe

PREFACE. I HPLC PRIMER. 1 Advantages and Disadvantages of HPLC. 1.1 How It
Works. 1.2 Other Ways to Make My Separation. 2 Selecting an HPLC System.
2.1 Characteristic Systems. 2.2 System Cost Estimates. 2.3 Columns. 3
Running Your Chromatograph. 3.1 Set-up and Start-up. 3.2 Sample Preparation
and Column Calibration. 3.3 Your First Chromatogram. II HPLC OPTIMIZATION.
4 Separation Models. 4.1 Partition. 4.2 Ion Exchange Chromatography. 4.3
Size Exclusion Chromatography. 4.4 Affinity Chromatography. 5 Column
Preparation. 5.1 Column Variations. 5.2 Packing Materials and Hardware. 5.3
Column Selection. 6 Column Aging, Diagnosis, and Healing. 6.1 Packing
Degrading--Bonded-Phase Loss. 6.2 Dissolved Packing Material--End Voids.
6.3 Bound Material. 6.4 Pressure Increases. 6.5 Column
Channeling--Center-Voids. 6.6 Normal Phase, Ion Exchange, and Size Columns.
6.7 Zirconium and Polymer Columns. 7 Partition Chromatography
Modifications. 7.1 Reverse-Phase and Hybrid Silica. 7.2 Acidic Phase
Silica. 7.3 Reverse-Phase Zirconium. 7.4 Partition Mode Selection. 8
"Nonpartition" Chromatography. 8.1 Ion Exchange. 8.2 Size Exclusion. 8.3
Affinity Chromatography. 9 Hardware Specifics. 9.1 System Protection. 9.2
Pumping. 9.3 Injectors and Autosamplers. 9.4 Detectors. 9.5 Fraction
Collectors. 9.6 Data Collection and Processing. 10 Troubleshooting and
Optimization. 10.1 Hardware and Tools--System Pacification. 10.2 Reverse
Order Diagnosis. 10.3 Introduction to Data Acquisition. 10.4 Solvent
Conservation. III HPLC UTILIZATION. 11 Preparative Chromatography. 11.1
Analytical Preparative. 11.2 Semipreparative. 11.3 "True" Preparative. 12
Sample Preparation and Methods Development. 12.1 Sample Preparation. 12.2
Methods Development. 12.3 Gradient Development. 13 Application Logics:
Separations Overview. 13.1 Fat-Soluble Vitamins, Steroid, and Lipids. 13.2
Water-Soluble Vitamins, Carbohydrates, and Acids. 13.3 Nucleomics. 13.4
Proteomics. 13.5 Clinical and Forensic Drug Monitoring. 13.6 Pharmaceutical
Drug Development. 13.7 Environmental and Reaction Monitoring. 13.8
Application Trends. 14 Automation. 14.1 Analog-to-Digital Interfacing. 14.2
Digital Information Exchange. 14.3 HPLC System Control and Automation. 14.4
Data Collection and Interpretation. 14.5 Automated Methods Development.
14.6 Data Exportation to the Real World. 15 Recent Advances in LC/MS
Separations. 15.1 A LC/MS Primer. 15.2 Microflow Chromatography. 15.3
Ultrafast HPLC Systems. 15.4 Chip HPLC Systems. 15.5 Standardized LC/MS in
Drug Design. 16 New Directions in HPLC. 16.1 Temperature-Controlled
Chromatography. 16.2 Ultrafast Chromatography. 16.3 Monolith Capillary
Columns. 16.4 Micro-Parallel HPLC Systems. 16.5 Two-Dimensional HPLC
Systems. 16.6 The Portable LC/MS. APPENDICES. APPENDIX A Personal
Separations Guide. APPENDIX B FAQs for HPLC Systems and Columns. APPENDIX C
Tables of Solvents and Volatile Buffers. APPENDIX D Glossary of HPLC Terms.
APPENDIX E HPLC Troubleshooting Quick Reference. APPENDIX F HPLC Laboratory
Experiments. Laboratory 1: System Start-up and Column Quality Control.
Laboratory 2: Sample Preparation and Methods Development. Laboratory 3:
Column and Solvent Switching and Pacification. Appendix G Selected
Reference List. INDEX.