Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.
Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.
Artikelnr. des Verlages: 12009473, 978-1-58829-562-0
2007
Seitenzahl: 272
Erscheinungstermin: 2. Februar 2007
Englisch
Abmessung: 241mm x 160mm x 22mm
Gewicht: 742g
ISBN-13: 9781588295620
ISBN-10: 1588295621
Artikelnr.: 22551175
Autorenporträt
Charles Z Hotz, Quantum Dot Corporation, Mountain View, CA, USA / Marcel Bruchez, Carnegie Mellon University, Pittsburgh, PA, USA
Inhaltsangabe
Imaging of Fixed Cells and Tissues.- Immunofluorescent Labeling of Proteins in Cultured Cells With Quantum Dot Secondary Antibody Conjugates.- Immunohistochemical Detection With Quantum Dots.- Quantum Dots for Multicolor Tumor Pathology and Multispectral Imaging.- Light and Electron Microscopic Localization of Multiple Proteins Using Quantum Dots.- Human Metaphase Chromosome FISH Using Quantum Dot Conjugates.- Imaging of Live Cells.- Biotin-Ligand Complexes With Streptavidin Quantum Dots for In Vivo Cell Labeling of Membrane Receptors.- Single Quantum Dot Tracking of Membrane Receptors.- Optical Monitoring of Single Cells Using Quantum Dots.- Peptide-Mediated Intracellular Delivery of Quantum Dots.- Multiple Cell Lines Using Quantum Dots.- Measuring Cell Motility Using Quantum Dot Probes.- Imaging of Live Animals.- Quantum Dots for In Vivo Molecular and Cellular Imaging.- Sentinel Lymph Node Mapping With Type-II Quantum Dots.- Macrophage-Mediated Colocalization of Quantum Dots in Experimental Glioma.- Flow Cytometry Applications.- Application of Quantum Dots to Multicolor Flow Cytometry.- Quantum Dots in Flow Cytometry.- Biochemical Applications.- Luminescent Biocompatible Quantum Dots.- Fluorescence-Based Analysis of Cellular Protein Lysate Arrays Using Quantum Dots.- Application of Quantum Dots to Multicolor Microarray Experiments.
Imaging of Fixed Cells and Tissues.- Immunofluorescent Labeling of Proteins in Cultured Cells With Quantum Dot Secondary Antibody Conjugates.- Immunohistochemical Detection With Quantum Dots.- Quantum Dots for Multicolor Tumor Pathology and Multispectral Imaging.- Light and Electron Microscopic Localization of Multiple Proteins Using Quantum Dots.- Human Metaphase Chromosome FISH Using Quantum Dot Conjugates.- Imaging of Live Cells.- Biotin-Ligand Complexes With Streptavidin Quantum Dots for In Vivo Cell Labeling of Membrane Receptors.- Single Quantum Dot Tracking of Membrane Receptors.- Optical Monitoring of Single Cells Using Quantum Dots.- Peptide-Mediated Intracellular Delivery of Quantum Dots.- Multiple Cell Lines Using Quantum Dots.- Measuring Cell Motility Using Quantum Dot Probes.- Imaging of Live Animals.- Quantum Dots for In Vivo Molecular and Cellular Imaging.- Sentinel Lymph Node Mapping With Type-II Quantum Dots.- Macrophage-Mediated Colocalization of Quantum Dots in Experimental Glioma.- Flow Cytometry Applications.- Application of Quantum Dots to Multicolor Flow Cytometry.- Quantum Dots in Flow Cytometry.- Biochemical Applications.- Luminescent Biocompatible Quantum Dots.- Fluorescence-Based Analysis of Cellular Protein Lysate Arrays Using Quantum Dots.- Application of Quantum Dots to Multicolor Microarray Experiments.
Rezensionen
From the reviews:
"...provides an excellent reference for protocols that can be utlized repeatedly to yield reliable performance from quantum dot-enabled applications." -Anticancer Research 27, May 2007
"Bruschez and Holtz have compiled an extremely useful set of applications and protocols in Quantum Dots, Applications in Biology. The protocols, written by the many leaders in QD imaging, cover a wide range of applications and provide detailed methodology for each application supported by excellent illustrations. This book certainly belongs on the shelf of anyone interested in biological imaging. ... in summary, this illustrative compilation of well-written protocols on QD is a must have for both the expert and novice interested in biological imaging." (Neel Ghosh, ChemBioChem, Issue 9, 2008)
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