Banafshe Larijani / Rudiger Woscholski / Colin Rosser (Hgg.)

Chemical Biology

Techniques and Applications
Ed. by Rudiger Woscholski, Colin Rosser and Banafshe Larijani

• Gebundenes Buch

Produktbeschreibung

Written by a team of international researchers and teachers at the cutting edge of chemical biology research, this book provides an exciting, comprehensive introduction to a wide range of chemical and physical techniques with applications in areas as diverse as molecular biology, signal transduction, drug discovery and medicine.

Techniques include: Cryo-electron microscopy, atomic force microscopy, differential scanning calorimetry in the study of lipid structures, membrane potentials and membrane probes, identification and quantification of lipids using mass spectroscopy, liquid state NMR, solid state NMR in biomembranes, molecular dynamics, two dimensional infra-red studies of biomolecules, single and two-photon fluorescence, optical tweezers, PET imaging and chemical genetics.

KEY FEATURES:
_ a unique guide to the rapidly evolving, interdisciplinary field of chemical biology.
_ adopts a molecular structure for maximum flexibility.
_ addresses relevant,topical chemical biological questions throughout.
_ includes stunning illustrations in full colour.
_ associates website with PowerPoint slides of figures within the book.

Chemical Biology: Techniques and Applications will provide an invaluable resource for final year undergraduate and post graduate bioscience and biomedical students and pharmaceutical researchers with an interest in this fascinating, and ever changing field.

Produktdetails

• Verlag: Wiley & Sons
• 1. Auflage
• Seitenzahl: 272
• Erscheinungstermin: 25. August 2006
• Englisch
• Abmessung: 248mm x 174mm x 22mm
• Gewicht: 680g
• ISBN-13: 9780470090640
• ISBN-10: 0470090642
• Artikelnr.: 20799653

Autorenporträt

Dr Larijani, London, UK.Research scientist, Cell Biophysics Laboratory, London Research Institute, Cancer Research UK, London WC2A 3PX Dr. Rudiger Woscholski, London, UK.Lecturer, Biochemistry Building, Imperial College, South Kensington, London SW7 2AY, UK Dr. Colin A. Rosser, Oxford, UK.Director of Studies, St Anthony School, Rye St Anthony, Headington, Oxford OX3 0BY, UK

Inhaltsangabe

Preface. List of Contributors. 1. Introduction. 1.1 Chemical biology - the
present. 1.2 Chemical biology - the past. 1.3 Chemical biology - the
future. 1.4 Chemical biology - mind the interdisciplinary gap. 1.5 An
introduction to the following chapters. 2. Cryomicroscopy. 2.1 The need for
(electron) microscopy. 2.2 Development of cryomicroscopy. 2.3
Sample-electron interaction. 2.4 Contrast in negatively stained and cryo
preparations. 2.5 Image formation. 2.6 Image analysis. 2.7 Software used in
the analysis of electron micrographs. 2.8 Examples. 2.9 Conclusions. 3.
Atomic force microscopy: applications in biology. 3.1 A brief history of
microscopy. 3.2 The scanning pribe microscope revolution. 3.3 The workings
of an AFM instrument. 3.4 Imaging biological molecules with force. 3.5
Factors influencing image quality. 3.6 Biological applications of AFM and
recent developments. 3.7 Conclusions and future directions. 4. Differential
scanning calorimetry in the study of lipid structures. 4.1 Introduction.
4.2 Membranes, lipids and lipid phases. 4.3 Heat exchanges and calorimetry.
4.4 Phase transitions in pure lipid-water systems. 4.5 Selected examples of
transitions in lipid mixtures. 4.6 Complex systems: lipid-protein mixtures
and cell membranes. 4.7 Conclusion. 5. Membrane potentials and membrane
probes. 5.1 Introduction: biological membranes; structure and electrical
properties. 5.2 Phospholipid membranes as molecular environments. 5.3 The
physical origins of the transmembrane ("¿), surface (ÆS) and dipolar (ÆD)
membrane potentials. 5.4 Measurement of membrane potentials. 5.5 Problems
with Spectroscopic Measurements of Membrane Potentials. 5.6 Spatial Imaging
of membrane potentials. 6. Identification and quantification of lipids
using mass spectrometry. 6.1 Introduction. 6.2 Lipid analysis by mass
spectrometry. 6.3 Conclusion. 7. Liquid-state NMR. 7.1 Introduction. 7.2
How NMR works: the basics. 7.3 Some NMR applications in biology. 7.4
Conclusion. 8. Solid-state NMR in biomembranes. 8.1 Introduction. 8.2 NMR
basics for membrane systems. 8.3 Applications of wide-line NMR to membrane
systems. 8.4 Applications of MAS to biomembranes and natural colloids. 8.5
Conclusion. 9. Molecular dynamics. 9.1 Introduction. 9.2 The basis of
molecular mechanics. 9.3 The basis of molecular dynamics. 9.4 Factors
affecting the length of simulations. 9.5 Problems caused by solvents. 9.6
How to build a lipid bilayer for simulation purposes. 9.7 Special cases of
membrane proteins. 9.8 Summary. 10. Two-dimensional infrared studies of
biomolecules. 10.1 Introduction. 10.2 Description of the technique. 10.3
Spectral simulations. 10.4 Two-dimensional studies of human lipoproteins.
10.5 Summary. 11. Biological applications of single- and two-photon
fluorescence. 11.1 Introduction. 11.2 Basic principles of fluorescence.
11.3 Main principles of RET via single-photon excitation. 11.4 Detection of
RET. 11.5 Biological examples of RET monitored by frequency-domain FLIM.
11.6 Two-photon fluorescence. 11.7 Applications of two-photon fluorescence.
11.8 Photoselection and fluorescence anisotropy. 11.9 Fluorescence
anisotropy and isotropic rotational diffusion. 11.10 Fluorescent probes in
proteins and membranes. 11.11 Future developments. 11.12 Conclusions. 12.
Optical tweezers. 12.1 Introduction. 12.2 Theoretical background. 12.3
Apparatus. 12.4 Data collection and analysis. 12.5 A biological
application. 12.6 Other biological examples. 12.7 Summary. 13. PET imaging
in chemical biology. 13.1 Introduction. 13.2 Positron emission tomography:
principles and instrumentation. 13.3 Applications of PET imaging in the
biomedical sciences. 13.4 Conclusions and outlook. 14. Chemical genetics.
14.1 Introduction. 14.2 Why chemicals?. 14.3 Chemical genetics - why now?.
14.4 The relationship between classical genetics and chemical genetics.
14.5 Forward chemical genetics. 14.6 Reverse chemical genetics. 14.7
Closing remarks. Index.

Rezensionen

"It is a very interesting and useful book, a good source of information (and, perhaps, inspiration!) and a good starting point for anyone who plans to venture deeper into the realm of chemical biology." ( The Biochemist , October 2008)