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The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately. Flow cytometry is key in this diagnostic pathway. This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of…mehr

Produktbeschreibung
The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately. Flow cytometry is key in this diagnostic pathway. This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams. The authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry. Practical Flow Cytometry in Haematology Diagnosis provides: * A clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditions * Carefully chosen real-life cases in each chapter, complemented by high quality morphological images * Help in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfalls All those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide.

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  • Produktdetails
  • Verlag: John Wiley & Sons
  • Seitenzahl: 256
  • Erscheinungstermin: 30. Januar 2013
  • Englisch
  • ISBN-13: 9781118487990
  • Artikelnr.: 37758461
Autorenporträt
Dr Mike Leach - Department of Haematology, Gartnavel General Hospital, Glasgow, UK Dr Mark Drummond - Haematology Department, Beatson West of Scotland Cancer Centre, Glasgow, UK Dr Allyson Doig - Department of Haematology, Gartnavel Hospital, Glasgow, U
Inhaltsangabe
Preface
vii Acknowledgements
viii 1 Introduction
1 References
2 2 Principles of Flow Cytometry
3 Introduction
3 Sample preparation
3 The flow cytometer
4 The fluidic system
4 The optical system
5 The electronic system
9 Data display
9 Acknowledgements
18 References
19 3 Limitations
20 Introduction
20 Clinical context issues
20 Sampling issues
20 Technical issues
25 Interpretation issues
28 Conclusion
29 References
29 4 Normal Blood and Bone Marrow Populations
31 Normal stem and precursor cell populations
31 References
41 5 Acute Leukaemia
43 Introduction
43 Identification of neoplastic precursor cells
45 Lineage assignment of acute leukaemia
52 Acute myeloid leukaemia
54 Precursor lymphoid neoplasms (acute lymphoblastic leukaemia/lymphoma)
77 Examination of cerebro-spinal fluid and serous effusions
88 Identification of genetic abnormalities by flow
92 Conclusion
93 References
93 6 Chronic Lymphoid Leukaemias and Exfoliating Lymphoma
100 Normal peripheral blood lymphoid populations
100 Identification of clonal lymphoid populations
101 Identification of clonal B-cell disorders
101 CD5 positive B-cell lymphoproliferative disorders
102 CD10 positive B-cell lymphoproliferative disorders
116 B-cell disorders identified using an extended B-cell panel
121 Identification of clonal T-cell disorders
125 CD4 positive T-cell disorders
128 CD8 positive T-cell disorders
136 CD4/CD8 positive T-cell disorders
141 CD4/8 negative disorders
141 Flow cytometric assessment of serous effusions
146 Flow cytometric assessment of cerebrospinal fluid
146 Summary
149 References
149 7 Myelodysplastic Syndromes and Myeloproliferative Neoplasms
152 Introduction
152 Myelodysplastic syndromes
152 Myelodysplastic/myeloproliferative neoplasms
163 Chronic myeloid leukaemia
165 The BCR-ABL negative myeloproliferative neoplasms
166 Mastocytosis
167 Summary
170 References
170 8 Disorders of Plasma Cells
173 Plasma cell disorders
173 Current diagnostic criteria
173 Plasma cell morphology
175 The applications of immunophenotyping to plasma cell disorders
178 References
183 9 Minimal Residual Disease
184 Introduction
184 Minimal residual disease analysis in the management of acute lymphoblastic leukaemia
187 Conclusions
199 Acknowledgements
200 References
200 10 Red Cells
Leucocytes and Platelets
202 Paroxysmal nocturnal haemoglobinuria
202 Red cell membrane disorders
208 Foetal maternal haemorrhage
211 Lymphocyte subset analysis and immunodeficiency
214 Haemopoietic stem cell enumeration
214 Granulocyte disorders
215 Platelet disorders
219 References
221 11 Reactive and Non-neoplastic Phenomena
225 Peripheral blood
225 Bone marrow
231 Pleural and pericardial fluids
236 Bronchoalveolar lavage specimens
238 Cerebrospinal fluid
238 References
240 Index
243
Rezensionen
"I would particularly recommend this book to haematology trainees, as an introduction to, but not as a replacement for, a period spent in an immunophenotyping laboratory." (British Journal of Haematology, 14 November 2014)

"There is no doubt that this book will find its way firmly onto the bookshelf in every laboratory, and into the work bags of many trainees." (Haem Trainee, 1 January 2014)

"This is a concise and very practical guide to the use of flow cytometry in hematological and hematopathological diagnosis. While not as detailed in immunophenotyping intricacies as other books of its kind, it shines with its practical approach and expert guidance applied to current clinical practice." (Doody's, 19 July 2013)