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Thermal Inactivation of Bacillus Anthracis Using Laser Irradiation of Micro-Etched Platforms
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The purpose of this research was to determine the probability of kill for a thermal inactivation strategy for use against biological agents; specifically the resilient endospore of Bacillus anthracis (Ba). The effort focused on short durations (milliseconds to several seconds) and temperatures (300 to 1300 K) simulating the periphery effects after an explosion generated by conventional munitions. For an improved statistical counting, applied microlithography techniques were used to produce micro-etched glass platforms consisting of 532 circular sample wells, evenly spaced, with dimensions of 5...
The purpose of this research was to determine the probability of kill for a thermal inactivation strategy for use against biological agents; specifically the resilient endospore of Bacillus anthracis (Ba). The effort focused on short durations (milliseconds to several seconds) and temperatures (300 to 1300 K) simulating the periphery effects after an explosion generated by conventional munitions. For an improved statistical counting, applied microlithography techniques were used to produce micro-etched glass platforms consisting of 532 circular sample wells, evenly spaced, with dimensions of 50 1/4m in diameter and approximately 3 1/4 in depth. Small carbon black radiators, which provide fast heating / cooling rate and confined temperature distribution, were produced by populating the etched wells with fine carbon particles of less than one 1/4m for good contact with the spores. In order to prevent the carbon black from oxidation at high temperatures in air, a multifunctional sol-gel coating was designed to cover both the hydrophilic glass surface and hydrophobic carbon surface. This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work was reproduced from the original artifact, and remains as true to the original work as possible. Therefore, you will see the original copyright references, library stamps (as most of these works have been housed in our most important libraries around the world), and other notations in the work. This work is in the public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work. As a reproduction of a historical artifact, this work may contain missing or blurred pages, poor pictures, errant marks, etc. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.
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