• Produktbild: Directed Enzyme Evolution
  • Produktbild: Directed Enzyme Evolution
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Directed Enzyme Evolution Screening and Selection Methods

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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

16.05.2003

Herausgeber

Frances H. Arnold + weitere

Verlag

Humana Press

Seitenzahl

383

Maße (L/B/H)

24,1/16/3 cm

Gewicht

839 g

Auflage

2003

Sprache

Englisch

ISBN

978-1-58829-286-5

Beschreibung

Rezension

"...covers a considerable number of protocols for a broad range of enzymes...very useful..." - ChemBioChem

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

16.05.2003

Herausgeber

Verlag

Humana Press

Seitenzahl

383

Maße (L/B/H)

24,1/16/3 cm

Gewicht

839 g

Auflage

2003

Sprache

Englisch

ISBN

978-1-58829-286-5

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: [email protected]

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  • Produktbild: Directed Enzyme Evolution
  • Produktbild: Directed Enzyme Evolution
  • Part I. Genetic Selections

    Genetic Complementation Protocols
    Jessica L. Sneeden and Lawrence A. Loeb

    Use of Pol I-Deficient E. coli for Functional Complementation of DNA Polymerase
    Manel Camps and Lawrence A. Loeb

    Selection of Novel Eukaryotic DNA Polymerases by Mutagenesis and Genetic Complementation of Yeast
    Ranga N. Venkatesan and Lawrence A. Loeb

    Autogene Selections
    Jijumon Chelliserrykattil and Andrew D. Ellington

    Selection for Soluble Proteins via Fusion with Chloramphenicol Acetyltransferase
    Volker Sieber

    Proside: A Phage-Based Method for Selecting Thermostable Proteins
    Andreas Martin, Franz X. Schmid, and Volker Sieber

    Minimization of Proteins by Random Fragmentation and Selection
    Gary W. Rudgers and Timothy Palzkill

    Part II. Screens for Enzymes

    Evaluating a Screen and Analysis of Mutant Libraries
    Oriana Salazar and Lianhong Sun

    Screening Mutant Libraries in Saccharomyces cerevisiae
    Thomas Bulter, Volker Sieber, and Miguel Alcalde

    Solid-Phase Screening Using Digital Image Analysis
    Alexander V. Tobias and John M. Joern

    Screening for Thermostability
    Patrick C. Cirino and Radu Georgescu

    High-Throughput Screening of Mutant a-Amylase Libraries for Increased Activity at 129°C
    Holger Berk and Robert J. Lebbink

    High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450
    Christopher R. Otey

    High-Throughput Screen for Aromatic Hydroxylation
    Christopher R. Otey and John M. Joern

    Colorimetric Screen for Aliphatic Hydroxylation by Cytochrome P450 Using p-Nitrophenyl-Substituted Alkanes
    Edgardo T. Farinas

    High-Throughput Screens Based on NAD(P)H Depletion
    Anton Glieder and Peter Meinhold

    High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidases
    Radu Georgescu

    Screenfor Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase
    Lianhong Sun and Makoto Yagasaki

    Colorimetric Dehydrogenase Screen Based on NAD(P)H Generation
    Kimberly M. Mayer

    Colorimetric Assays for Screening Laccases
    Miguel Alcalde and Thomas Bulter

    pH Sensing Agar Plate Assays for Esterolytic Enzyme Activity
    Karl E. Griswold

    A pH-Indicator-Based Screen for Hydrolytic Haloalkane Dehalogenase
    Huimin Zhao

    Detection of Aromatic a-Hydroxyketones with Tetrazolium Salts
    Michael Breuer and Bernhard Hauer

    Selection of Heat-Stable Clostridium cellulovorans Cellulases After In Vitro Recombination
    Koichiro Murashima and Roy H. Doi

    Screening and Selection Strategies for Disulfide Isomerase Activity
    Ronald Lafond, Xiaoming Zhan, and George Georgiou

    An Overview of High-Throughput Screening Systems for Enantioselective Enzymatic Transformations
    Manfred T. Reetz

    Select Protocols of High-Throughput ee-Screening Systems for Assaying Enantioselective Enzymes
    Manfred T. Reetz

    Directed Evolution of the Substrate Specificities of a Site-Specific Recombinase and an Aminoacyl-tRNA Synthetase Using Fluorescence-Activated Cell Sorting (FACS)
    Stephen W. Santoro and Peter G. Schultz

    Calmodulin-Tagged Phage and Two-Filter Sandwich Assays for the Identification of Enzymatic Activities
    Christian Heinis, Julian Bertschinger, and Dario Neri

    High-Throughput FACS Method for Directed Evolution of Substrate Specificity
    Mark J. Olsen, Jongsik Gam, Brent L. Iverson, and George Georgiou

    Improving Protein Folding Efficiency by Directed Evolution Using the GFP Folding Reporter
    Geoffrey S. Waldo

    Index