Comparative Transcriptome Analysis using cDNA¿AFLP Technique
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Comparative Transcriptome Analysis using cDNA¿AFLP Technique

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An increased understanding of the molecular mechanisms involved in establishment and development of entomopathogenic fungi can be regarded as an important step towards their effective future insect pest management. To this end, I have successfully applied two different PCR-based approaches - cDNA-AFLPs and Quantitative real-time PCR analysis to identify B. bassiana genes putatively involved in overcoming host insect specific barriers. The cDNA-AFLP display proved to be a very powerful transcriptome-wide gene discovery tool in B. bassiana just as with many other organisms in which this approach...