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Band 27

Biomembrane Protocols II. Architecture and Function

Aus der Reihe Methods in Molecular Biology

97,99 €

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Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

11.09.2013

Verlag

Humana Press

Seitenzahl

362

Maße (L/B/H)

22,9/15,2/2,1 cm

Gewicht

549 g

Auflage

1994

Sprache

Englisch

ISBN

978-1-4899-3909-8

Beschreibung

Rezension

...the editors have assembled an authoritative set of reviews, which give generally clear practical guidance and useful methodological tips. -SGM Quarterly

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

11.09.2013

Verlag

Humana Press

Seitenzahl

362

Maße (L/B/H)

22,9/15,2/2,1 cm

Gewicht

549 g

Auflage

1994

Sprache

Englisch

ISBN

978-1-4899-3909-8

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: [email protected]

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  • Produktbild: Biomembrane Protocols
  • Produktbild: Biomembrane Protocols

  • Crystallization of Membrane Proteins for X-Ray Analysis. Determination of Cell Surface Polarity by Solid-Phase Lactoperoxidase Iodination. Biochemical Methods to Determine Cell Surface Topography: Part A: Labeling of Oxidized Glycoproteins with 3H-Borohydride; Part B: Identification and Separation of Integral Membrane Proteins Using Triton X-114; Part C: Surface-Selective Labeling of Polarized Proteins on Tight Epithelia Using Sulfo-N-Hydroxysuccinimido-Biotin (S-NHS-B). Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins: Part A: Preparation of Antibodies; Part B: Affinity-Purification of Antipeptide Antibodies; Part C: Purification of Membrane Proteins by Immunoaffinity Chromatography; Part D: Competitive ELISA for Determining Membrane Protein Topology. The Production of Monoclonal Antibodies to Membrane Proteins: Part A: Production of Hybridoma Cell Lines; Part B: Screening for Monoclonal Antibody Production. Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles. Measurement of Protein-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy. Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy. Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as Probes. Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Proteins as Tools. Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry. Fluorescent Glycerolipid Probes: Synthesis and Use for Examining Intracellular Lipid Trafficking: Part A: Synthesis of Fluorescent Glycerolipid Probes; Part B: Delivery of Fluorescent Glycerophospholipid Probes to Cultured Cells. Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids. Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes Extraction and Assay of Cyclic Nucleotides. Analysis of G-Proteins Regulating Signal Transduction Pathways: Part A: Identification of G-Proteins; Part B: GTPase Studies; Part C: Reconstitution of Second Messenger Pathways in Permeabilized Cell Preparations Using GTP Analogs and Receptor Ligands. Assay of Protein Kinases and Protein Phosphorylation: Part A: Protein Kinase-Mediated Phosphorylation Events; Part B: Measurement of Protein Kinase Expression; Part C: Measurement of Protein Kinase Activity and Translocation. Analysis of Cellular Phosphoinositides and Phosphoinositols: Extraction and Simple Analytical Procedures: Part A: Biosynthesis and Extraction of Phosphoinositides and Phosphoinositols; Part B: Separation of Phosphoinositols by Anion-Exchange Chromatography; Part C: Separation of Phosphoinositides by Thin-Layer Chromatography; Part D: Deacylation of Phospholipids and Separation of Products by Anion-Exchange Chromatography. Analysis of Cellular Phosphoinositides and Phosphoinositols by High Performance Liquid Chromatography: Part A: Sample and Standards Preparation; Part B: HPLC Analytical Methods. Cytosolic Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes. Membrane Permeabilization with Bacterial Toxins. Measurement of Ion Fluxes and pH Gradients Across Cell Membranes. Ligand Binding and Processing: The Perfused Liver as a Model System. The Binding of Protein-Ligands to Cell-Surface Receptors. Appendix: 1. Density Gradient Media. Appendix: 2. Balanced Salt Solutions. Index.