Novel Biosensors Using Intact Liposome Microarrays
Many of the interactions studied in the biological and biomedical
sciences occur with receptors at cell membrane surfaces. Prominent
examples are neurotransmitters, cytokine receptors, tyrosine kinase
receptors, ligang-and voltage-gated ion channels, G protein-coupled
receptors, and antibody receptors. Interactions with these
receptors are of special importance not only to academics, but also
to the pharmaceutical industry as almost half of the 100
best-selling drugs on the market are targeted to a membrane
receptor. More than 50% of current drug targets are membrane
bound.Naturally, there is a great interest in development of
sensors using membrane proteins for drug discovery and
high-throughput screening for detection of pathogens/toxins. This
book describes protocols to array individual, intact small
unilamellar vesicles (liposomes) onto chemically modified microwell
substrates. These arrays can be used as biosensors using membrane
proteins or receptors incorporated in the lipid bilayer of the
arrayed liposomes. One of the key examples covered in this work is
the use of this platform to display GM1 ganglioside receptor to
detect cholera toxin in analyte solutions.
Dr. Nikhil D Kalyankar received Bachelor of Chemical Engineering at UDCT Mumbai in 2002 and PhD in Chemical Engineering at The City University of New York in 2007. His research interests include Biosensors, Surface and Interfacial Science, Nanotechnology, Green Energy, Semiconductors etc. He is also a private pilot and lives in San Jose CA.
