Adipose Tissue Protocols

Adipose Tissue Protocols

This revised book reflects the radical expansion and revision of techniques and experimental approaches employed in the study of adipose tissue. Several updated chapters are combined with many new contributions from internationally recognized experts.

The radical expansion and revision of techniques and experimental approaches employed in the study of adipose tissue are reflected in this revised book. Several updated chapters are combined with many new contributions from internationally recognized experts. Conventional and state-of-the-art methodologies are covered, as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. This revised all-in-one manual is essential reading for newcomers to this burgeoning area as well as a valuable volume for more experienced researchers.

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Inhaltsangabe

Introduction.
Current Microarray Surface Chemistries.
Non
Fouling Surfaces.
Optimization of Oligonucleotide DNA Microarray.
Detection of DNA Copy Number Alterations in Complex Genomes Using Array CGH.
Evaluating the Quality of Data Microarray Measurements.
Construction of Oligonucleotide Microarrays (Biochip) Using Heterobifunctional Reagents.
Choice of Polymer Matrix, its Functionalization and Estimation of Functional Group Density for Preparation of Biochips.
Methods in High
Resolution, Array
Based Comparative Genomic Hybridization.
Design and Fabrication of Spotted Long Oligonucleotide Microarrays for Gene Expression.
In
Situ Oligonucleotide Arrays on Plastic.
Detecting Ligated Fragments on Oligonucleotide Microarrays
Optimizing Chip Design, Array MLPA Modification and Hybridization Parameters.
Detection of SNPs in Cancer Related Genes by Minisequencing on a Microelectronic DNA Chip.
Hybridization Analysis Using Oligonucleotide Probe Arrays.
In situ Synthesis of Peptide Microarrays Using Ink
Jet Microdispensing.
Intein
Mediated Peptide Arrays for Epitope Mapping and Kinase/Phosphatase Assays.
Printing Low Density Protein Arrays in Microplates.
Protein Microarray Applications
Forward and Reverse Protein Arrays.
Cell Microarray for Functional Exploration of Genomes.
Quantification of Mixed
phase Hybridization on Polymer Microparticles by Europium (III) Ion Fluorescence.
Measurement of the Sugar
Binding Specificity of Lectins Using Multiplexed Bead
Based Suspension Arrays.
Nanotechnology.